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Insights into the Global Transcriptome Response of Lentinula edodes Mycelia during Aging

文献类型： 外文期刊

作者： Gao, Qi 1 ; Fan, Yangyang 1 ; Wei, Sai 1 ; Song, Shuang 1 ; Guo, Yuan 1 ; Wang, Shouxian 1 ; Liu, Yu 1 ; Yan, Dong 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Engn Res Ctr Edible Mushroom, 9 Shuguang Garden Zhonglu, Beijing 100097, Peoples R China

2.Beijing Univ Agr, Coll Plant Sci & Technol, 7 Beinong Rd, Beijing 102208, Peoples R China

关键词： Shiitake mushroom; mycelial senescence; WGCNA; autophagy; mitophagy

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 9 卷 3 期

页码：

收录情况： SCI

摘要： The spawn of Lentinula edodes and other basidiomycete fungi tend to age with long-term culture. This causes heavy yield losses if aging spawn is used for propagation. In this study, we cultivated dikaryotic L. edodes mycelia in plates for 60 days to produce intrinsic aging phenotypes. We found that intracellular reactive oxygen species levels increased in contrast to mitochondrial depolarization and also observed greater DNA fragmentation with longer culture time. Transcriptome analysis of mycelia at different growth stages revealed pronounced expression differences between short- and long-term cultures. In particular, "phenylalanine, tyrosine, and tryptophan biosynthesis", "mitophagy and autophagy", "MAPK signaling pathway", and "ABC transporter" were among the enriched terms in the mycelial aging process. Weighted correlation network analysis identified LeAtg8, LeHog1, LePbs2, and LemTOR as key genes during aging. Western blotting confirmed that LeATG8 and phosphorylated LeHOG1 protein levels were significantly upregulated in aging mycelia. Our combined analytical approach provides insights into the mechanisms that regulate mycelial aging, indicating that autophagy/mitophagy plays a major role in counteracting the effects of age on mycelial growth development.

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