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Antibacterial Activity and Mechanism of Polygonatum sibiricum Extract Against Bacillus cereus and Its Application in Pasteurized Milk

文献类型： 外文期刊

作者： Fei, Peng 1 ; Sun, Zongyu 1 ; Liu, Xinyu 1 ; Jiang, Peiyi 1 ; Feng, Hongxia 3 ; Chen, Xi 4 ; Ma, Yan 1 ; Dong, Gege 1 ; Fan, Chengwei 1 ; Bai, Mengyang 1 ; Li, Yadi 1 ; Chang, Yunhe 3 ;

作者机构： 1.Nanyang Inst Technol, Sch Zhang Zhongjing Hlth Care & Food, Nanyang, Peoples R China

2.Zhongyuan Food Lab, Luohe, Peoples R China

3.Guiyang Univ, Food & Pharmaceut Engn Inst, Guiyang 550005, Peoples R China

4.Guizhou Acad Agr Sci, Inst Integrated Agr Dev Res, Guiyang, Peoples R China

关键词： Polygonatum sibiricum extract; Bacillus cereus; antibacterial activity; antibacterial mechanism; pasteurized milk

期刊名称：FOODBORNE PATHOGENS AND DISEASE （ 2022影响因子：2.8； 五年影响因子：3.2 ）

ISSN： 1535-3141

年卷期： 2024 年 21 卷 3 期

页码：

收录情况： SCI

摘要： The purpose of this study was to reveal the antibacterial activity and mechanism of Polygonatum sibiricum extract (PSE) against Bacillus cereus and further analyze the application of PSE in pasteurized milk (PM). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values and growth curve analysis were used to evaluate the antibacterial activity of PSE against B. cereus. The changes in contents of intracellular adenosine 5 ' -triphosphate (ATP) and reactive oxygen species (ROS), activities of beta-galactosidase, adenosine triphosphatase (ATPase) and alkaline phosphatase (AKP), cell membrane potential, protein and nucleic acid leakage, and cell morphology were used to reveal the antibacterial mechanism. The effects of PSE on viable count and sensory evaluation of PM during storage were analyzed. The results showed that the MIC and MBC values of PSE against B. cereus were 2 and 4 mg/mL, respectively. Growth curve analysis showed that PSE with a concentration of 2 MIC could completely inhibit the growth of B. cereus. After treatments with PSE, the levels of intracellular ATP and ROS, and activities of beta-galactosidase, ATPase and AKP of B. cereus were significantly reduced (p < 0.05). Cell membrane was depolarized, amounts of protein and nucleic acid leakage were significantly increased (p < 0.05), and cell morphology was destroyed. Furthermore, PSE significantly reduced the viable count of B. cereus in PM and improved the sensory quality of PM during storage (p < 0.05). Together, our findings suggested that PSE had the desired effect as a natural preservative applied in PM.

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