文献类型： 外文期刊

作者： Cheng, L. ¹ ; Zhu, H. X. ¹ ; Wei, Y. H. ¹ ; Guo, L. Z. ¹ ; Weng, H. ¹ ; Guo, Q. Y. ¹ ;

作者机构： 1.Qinghai Univ, Acad Agr & Forestry Sci, Xining 810016, Peoples R China

2.Key Lab Agr Integrated Pest Management Qinghai Pr, Xining 810016, Peoples R China

3.Qinghai Acad Agr & Forestry Sci, Inst Plant Protect, Xining 810016, Peoples R China

关键词： Endophytic fungi; Weeds; Herbicidal activity; Crop safety; Fermentation; Optimization; Formulation

期刊名称：JOURNAL OF PLANT DISEASES AND PROTECTION （ 影响因子：1.928； 五年影响因子：1.652 ）

ISSN： 1861-3829

年卷期： 2021 年 128 卷 6 期

页码：

收录情况： SCI

摘要： Weeds are one of the main problems faced by agronomists, which are the cause of large losses of crops in many farmlands. Screening of novel biocontrol agents against weeds is highly desired to control a wide variety of weeds. The endophytic fungal isolate HL-1 was isolated from the phyllosphere of Cirsium setosum indigenous to agricultural fields of Hualong, Qinghai Province. The isolate was evaluated for its herbicidal activity against target weeds and its safety to major crops. The feasibility of solid substrate fermentation for the spore production of isolate HL-1 has been examined. The results indicated that the disease incidences of Galium aparine, Chenopodium album, Malva crispa, and Polygonum lapathifolium applied with HL-1 filtrate were 96.24%, 87.48%, 55.71%, and 65.48%, and the fresh weight reductions were 69.04%, 72.02%, 66.12%, and 58.03%, respectively. The filtrate was safe to Triticum aestivum and Vicia faba and slightly pathogenic to Brassica napus, Pisum sativum, and Hordeum vulgare. The strain was validated as Alternaria alternata by cultural characteristic and internal transcribed spacer sequencing. Optimization of the carbon and nitrogen sources for cultural media and materials for solid-state fermentation demonstrated that strain HL-1 had better colony growth and spore yield with the optimal carbon source of glucose, nitrogen source of yeast extract, and the optimal material was rapeseed cake. The optimal fermentation conditions of HL-1 are a temperature of 25 celcius, an inocula age of 72 h, initial moisture content of 40%, and an inoculated quantity of 8%. Its formulation showed a strong herbicidal activity on the tested weeds in both pot and field experiments, especially Stellaria media. Thus, phyllosphere microbiota is considered to be an important resource for implementing new biological herbicides for weed control, and this work provides a formulation of a mycoherbicide made up of spores produced by HL-1.