文献类型： 外文期刊

作者： Yang, Li-Rong ¹ ; Qiang, Xiao ² ; Zhang, Bao-Qin ¹ ; Tang, Mei-Jun ² ; Zhang, Chuan-Xi ¹ ;

作者机构： 1.Zhejiang Univ, Inst Insect Sci, Hangzhou 310029, Zhejiang, Peoples R China

2.Chinese Acad Agr Sci, Res Inst, Hangzhou 310000, Zhejiang, Peoples R China

3.Henan Acad Agr Sci, Zhengzhou 450002, Peoples R China

关键词： Iragoidae fasciata;nucleopolyhedrovirus;SNPV;polyhedrin region;phylogeny;permissive cell line

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

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收录情况： SCI

摘要： The tea slug moth Iragoidae fasciata (Lepidoptera, Eucleidae) is one of the main insect pests that attacktea bushes. A new nucleopolyhedrovirus (NPV) called Iragoidae fasciata NPV (IrfaNPV) was recently iso-lated from diseased larvae. An 11,626 bp fragment of the viral genomic DNA containing the polyhedringene and other 12 genes was cloned and sequenced. Gene comparison and phylogenetic analysis showedthat IrfaNPV is a member of the Group I NPVs. However, the genomic organization of IrfaNPV is highlydistinct. In addition, electron microscopy analysis showed that IrfaNPV is a single nucleocapsid NPV(SNPV). An inoculation assay showed that IrfaNPV is semi-permissive in the Trichoplusia ni cell lineTn-5B-4. Bioassays on lethal concentration (LCso) and lethal time (LTso) were conducted to test the sus-ceptibility of I. fasciata larvae to the virus.