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Quercetin Inhibits Hsp70 Blocking of Bovine Viral Diarrhea Virus Infection and Replication in the Early Stage of Virus Infection

文献类型： 外文期刊

作者： Chen, Nannan 1 ; Liu, Yu 1 ; Bai, Tongtong 1 ; Chen, Jinwei 1 ; Zhao, Zhibo 1 ; Li, Jing 1 ; Shao, Baihui 1 ; Zhang, Zecai 1 ; Zhou, Yulong 1 ; Wang, Xue 1 ; Zhu, Zhanbo 1 ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Bovine Dis Control Northeast China, Daqing 163319, Peoples R China

3.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar 161006, Peoples R China

4.Heilongjiang Bayi Agr Univ, Heilongjiang Prov Engn Res Ctr Prevent & Control, Daqing 163319, Peoples R China

关键词： BVDV; flaviviruses; antiviral; quercetin; Hsp70; inhibitor; oxidative stress

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 11 期

页码：

收录情况： SCI

摘要： Bovine viral diarrhea virus (BVDV), a positive-strand RNA virus of the genus Pestivirus in the Flaviviridae family, is the causative agent of viral diarrheal disease in bovine. BVDV has been used as a surrogate model for the hepatitis C virus (HCV) to evaluate the efficacy of antiviral drugs. The plant flavonol quercetin causes multiple health-promoting effects in humans and animals. It can be made into a variety of additives, and it exerts a variety of immunomodulatory effects with the potential to be used as an antiviral agent. However, quercetin's antiviral effect and mechanism of action on BVDV are still unclear. Therefore, this study was designed to evaluate quercetin's effect on BVDV virus replication in vitro and in vivo and elucidate its mechanism of action. A CCK-8 kit was used to analyze the toxicity of the quercetin to the MDBK cells. Western blot, qRT-PCR, TCID50, and histological analysis were used to determine the mechanism of quercetin's anti-BVDV activity. An oxidative stress kit was used to evaluate the effects of quercetin on ROS, antioxidant enzymes, and MDA indexes. The effect of quercetin on IL-2 and IFN-gamma in the serum of mice was determined by using an ELISA kit. The results showed that quercetin inhibits Hsp70, blocks BVDV infection in the early stage of virus infection and inhibits BVDV replication by inhibiting oxidative stress or ERK phosphorylation. In addition, quercetin alleviated the decrease in IFN-gamma and IL-2 in the serum of BVDV-infected mice. Quercetin ameliorated BVDV-induced histopathological changes. In summary, this study demonstrated for the first time the role of Hsp70 in BVDV infection and the potential application of quercetin in treating BVDV infection.

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