文献类型： 外文期刊

作者： Chen, Yun-long ¹ ; Nie, Hao-nan ¹ ; Dong, Hui ¹ ; Gao, Qi ¹ ; Peng, Xue ¹ ; Wang, Ning ¹ ; Chen, Xueling ⁴ ; Liu, Qing-zheng ⁶ ; Li, Jiang-kuo ² ; Xu, Xiang-bin ³ ; Xue, You-lin ¹ ;

作者机构： 1.Liaoning Univ, Coll Light Ind, Shenyang 110036, Peoples R China

2.Tianjin Acad Agr Sci, Natl Engn & Technol Res Ctr Preservat Agr Prod Tia, Tianjin 300384, Peoples R China

3.Hainan Univ, Coll Food Sci & Engn, Haikou 570228, Peoples R China

4.Hubei Acad Agr Sci, Inst Farm Prod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China

5.Party Sch Liaoning Prov Party Comm, Dept Reg Econ Dev, Shenyang 110161, Peoples R China

6.Xinghua Lianfu Foods Co Ltd, Xinghua 225700, Peoples R China

7.Liaoning Univ, Coll Light Ind, 66 Chongshan Middle Rd, Shenyang, Liaoning, Peoples R China

关键词： Dioscorin; & gamma;-Aminobutyric acid; Emulsifying properties; Freeze-drying; Spray-drying; Molecular dynamics simulation

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）

ISSN： 0963-9969

年卷期： 2023 年 171 卷

页码：

收录情况： SCI

摘要： Many studies have shown that ?-aminobutyric acid (GABA) exhibits various beneficial biological activities, including gut-modulating, neuro-stimulating, and cardio-protecting activities. Naturally, GABA exists in small amounts in yam, which is primarily synthesized by the decarboxylation of L-glutamic acid in the presence of glutamate decarboxylase. Dioscorin, the major tuber storage protein of yam, has been shown to have good solubility and emulsifying activity. However, how GABA interacts with dioscorin and affects their properties has yet to be clarified. In this research, the physicochemical and emulsifying properties of GABA-fortified dioscorin, which was dried by spray drying and freeze drying, were studied. As results, the freeze-dried (FD) dioscorin produced more stable emulsions, while the spray-dried (SD) dioscorin adsorbed more rapidly to oil/water (O/W) interface. The fluorescence spectroscopy, ultraviolet spectroscopy and circular dichroism spectroscopy showed that GABA changed the structure of dioscorin, by exposing its hydrophobic groups. The addition of GABA significantly promoted the adsorption of dioscorin to the O/W interface and prevented droplets coalescence. The results of molecular dynamics simulation (MD) showed that GABA destroyed the H-bond network between dioscorin and water, increased surface hydrophobicity and finally improved the emulsifying properties of dioscorin.