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Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat

文献类型： 外文期刊

作者： Liu, Caiyun 1 ; Guo, Wei 1 ; Wang, Yang 2 ; Fu, Bisheng 1 ; Dolezel, Jaroslav 3 ; Liu, Ying 1 ; Zhai, Wenling 1 ; Said, Mahmoud 3 ; Molnar, Istvan 3 ; Holusova, Katerina 3 ; Zhang, Ruiqi 2 ; Wu, Jizhong 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Jiangsu Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Agron, Natl Key Lab Crop Genet & Germplasm Enhancement, JCIC MCP, Nanjing 210095, Jiangsu, Peoples R China

3.Inst Expt Bot, Ctr Hana Reg Biotechnol & Agr Res, Slechtitelu 31, CZ-783671 Olomouc, Czech Republic

4.Agr Res Ctr, Field Crops Res Inst, 9 Gamma St, Cairo 12619, Egypt

5.ELKH, Agr Inst, Ctr Agr Res, H-2462 Martonvasar, Hungary

6.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Yangzhou 225009, Jiangsu, Peoples R China

7.Zhongshan Biol Breeding Lab, Nanjing 210014, Jiangsu, Peoples R China

关键词： Triticum aestivum; Rhizoctonia cerealis; Dasypyrum villosum; Flow sorting; KASP

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 5 期

页码：

收录情况： SCI

摘要： Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven, has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat-Dasypyrum villosum T2DS.2V#4L translocation line in the background of Chinese Spring (CS) showed stable resistance to R. cerealis. Introgression of the T2DS.2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS.2V#4L in comparison with NIL-T2DS.2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3, and BC2F2:4 generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and 8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and 2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and 30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat back-grounds. T2DS.2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.(c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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