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Genome-wide characterization of Histone gene family and expression profiling during microspore development in radish (Raphanus sativus L.)

文献类型： 外文期刊

作者： Wang, Qijiao 1 ; Fan, Lianxue 1 ; Su, Xiaojun 2 ; Ying, Jiali 1 ; Xu, Liang 1 ; Li, Cui 1 ; Wang, Yan 1 ; Liu, Liwang 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Natl Key Lab Crop Genet & Germplasm Enhancement, Key Lab Hort Crop Biol & Genet Improvement East C, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vegetable Sci, Nanjing 210014, Jiangsu, Peoples R China

3.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Histone gene; Raphanus sativus L; RsCENH3; RT-qPCR; Expression profile

期刊名称：GENE （ 影响因子：3.913； 五年影响因子：3.48 ）

ISSN： 0378-1119

年卷期： 2022 年 815 卷

页码：

收录情况： SCI

摘要： Histone, a predominant protein component of chromatin, participates in DNA packaging and transcriptional regulation. However, the available information of Histone gene family is limited in radish. In this study, a total of 42 Histone gene family members were identified from the radish genome. Sequence alignment and phylogenetic analyses classified the Histone family into three groups (H2A, H2B and H3). Motif analysis showed that the functions of some motifs shared by H3 subfamily genes were related to chromosome regulation and cell development activities, such as motif 5 containing Cks1 and PPR region. Analysis of intron/exon structure indicated that RsCENH3 (RsHistone 18) has the characteristics of variant Histone. Furthermore, several motifs, including the LTR, G-box and TC-elements, were found in the promoters of RsHistone genes, which involved in cell development or various abiotic stresses responses. Transcriptome analysis indicated that the RsHistone genes exhibited higher expression level in floral buds than in roots and leaves. Subcellular localization showed that the RsCENH3 was localized on the nucleus, and it was highly expressed in the floral bud of 3.0-4.0 mm in radish. These findings would provide valuable information for characterization and potential utilization of Histone genes, and facilitate the efficient induction of double haploid plants in radish.

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