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A spontaneous thermo-sensitive female sterility mutation in rice enables fully mechanized hybrid breeding

文献类型： 外文期刊

作者： Li, Haoxuan 1 ; You, Chenjiang 5 ; Yoshikawa, Manabu 6 ; Yang, Xiaoyu 7 ; Gu, Haiyong 9 ; Li, Chuanguo 9 ; Cui, Jie 7 ; Chen, Xuemei 5 ; Ye, Nenghui 4 ; Zhang, Jianhua 1 ; Wang, Guanqun 1 ;

作者机构： 1.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Peoples R China

2.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Peoples R China

3.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China

4.Hunan Agr Univ, Coll Agr, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China

5.Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA

6.Natl Agr & Food Res Org, Inst Agrobiol Sci, Div Plant & Microbial Sci, 2-1-2 Kannondai, Tsukuba, Ibaraki, Japan

7.Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Prov Key Lab Plant Epigenet, Shenzhen, Guangdong, Peoples R China

8.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R China

9.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou, Guangdong, Peoples R China

期刊名称：CELL RESEARCH （ 影响因子：46.297； 五年影响因子：38.109 ）

ISSN： 1001-0602

年卷期： 2022 年 32 卷 10 期

页码：

收录情况： SCI

摘要： Male sterility enables hybrid crop breeding to increase yields and has been extensively studied. But thermo-sensitive female sterility, which is an ideal property that may enable full mechanization in hybrid rice breeding, has rarely been investigated due to the absence of such germplasm. Here we identify the spontaneous thermo-sensitive female sterility 1 (tfs1) mutation that confers complete sterility under regular/high temperature and partial fertility under low temperature as a point mutation in ARGONAUTE7 (AGO7). AGO7 associates with miR390 to form an RNA-Induced Silencing Complex (RISC), which triggers the biogenesis of small interfering RNAs (siRNAs) from TRANS-ACTING3 (TAS3) loci by recruiting SUPPRESSOR OF GENE SILENCING (SGS3) and RNA-DEPENDENT RNA POLYMERASE6 (RDR6) to TAS3 transcripts. These siRNAs are known as tasiR-ARFs as they act in trans to repress auxin response factor genes. The mutant TFS1 (mTFS1) protein is compromised in its ability to load the miR390/miR390* duplex and eject miR390* during RISC formation. Furthermore, tasiR-ARF levels are reduced in tfs1 due to the deficiency in RDR6 but not SGS3 recruitment by mTFS1 RISC under regular/high temperature, while low temperature partially restores mTFS1 function in RDR6 recruitment and tasiR-ARF biogenesis. A miR390 mutant also exhibits female sterility, suggesting that female fertility is controlled by the miR390-AGO7 module. Notably, the tfs1 allele introduced into various elite rice cultivars endows thermo-sensitive female sterility. Moreover, field trials confirm the utility of tfs1 as a restorer line in fully mechanized hybrid rice breeding.

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