RAD-Seq-Based High-Density Linkage Maps Construction and Quantitative Trait Loci Mapping of Flowering Time Trait in Alfalfa (Medicago sativa L.)
文献类型： 外文期刊
作者： Jiang, Xueqian 1 ; Yang, Tianhui 2 ; Zhang, Fan 1 ; Yang, Xijiang 1 ; Yang, Changfu 1 ; He, Fei 1 ; Long, Ruicai 1 ; Gao, Ting 2 ; Jiang, Yiwei 3 ; Yang, Qingchuan 1 ; Wang, Zhen 1 ; Kang, Junmei 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan, Peoples R China
3.Purdue Univ, Dept Agron, W Lafayette, IN USA
关键词： alfalfa; flowering time; genetic map; QTL; RAD-seq; RNA-seq
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Alfalfa (Medicago sativa L.) is a perennial forage crop known as the "Queen of Forages." To dissect the genetic mechanism of flowering time (FT) in alfalfa, high-density linkage maps were constructed for both parents of an F1 mapping population derived from a cross between Cangzhou (P1) and ZhongmuNO.1 (P2), consisting of 150 progenies. The FT showed a transgressive segregation pattern in the mapping population. A total of 13,773 single-nucleotide polymorphism markers was obtained by using restriction-site associated DNA sequencing and distributed on 64 linkage groups, with a total length of 3,780.49 and 4,113.45 cM and an average marker interval of 0.58 and 0.59 cM for P1 and P2 parent, respectively. Quantitative trait loci (QTL) analyses were performed using the least square means of each year as well as the best linear unbiased prediction values across 4 years. Sixteen QTLs for FT were detected for P1 and 22 QTLs for P2, accounting for 1.40-16.04% of FT variation. RNA-Seq analysis at three flowering stages identified 5,039, 7,058, and 7,996 genes that were differentially expressed between two parents, respectively. Based on QTL mapping, DEGs analysis, and functional annotation, seven candidate genes associated with flowering time were finally detected. This study discovered QTLs and candidate genes for alfalfa FT, making it a useful resource for breeding studies on this essential crop.
- 相关文献
作者其他论文 更多>>
-
Plant and soil responses to tillage practices change arbuscular mycorrhizal fungi populations during crop growth
作者：Li, Jing;Jia, Lijuan;Wang, Zhen;Ji, Lei;Jin, Ke;Li, Jing;Xu, Zhuwen;Struik, Paul C.;An, Zhengfeng;Yao, Yuqing;Lv, Junjie;Zhou, Tao
关键词：tillage practices; arbuscular mycorrhizal fungi; wheat growth stages; co-occurrence; Loess Plateau
-
FtsH proteases confer protection against salt and oxidative stress in Medicago sativa L.
作者：Li, Mingna;Zhu, Xiaoxi;Yu, Qianwen;Yu, Andong;Chen, Lin;Kang, Junmei;Wang, Xue;Yang, Qingchuan;Long, Ruicai;Yang, Tianhui
关键词：Alfalfa; Phylogenetic relationship; Abiotic stress tolerance; ROS scavenge; RS signaling
-
A genome-wide association study reveals novel loci and candidate genes associated with plant height variation in Medicago sativa
作者：Jiang, Xueqian;He, Fei;Zhang, Fan;Jiang, Xu;Long, Ruicai;Li, Mingna;Yang, Qingchuan;Kang, Junmei;Zhang, Tiejun;Yang, Tianhui;Wang, Chuan;Gao, Ting;Yang, Tianhui;Wang, Yue
关键词：Alfalfa (Medicago sativa); GWAS; Plant height; Phosphate transporter
-
Transcriptomic analysis reveals that methyl jasmonate confers salt tolerance in alfalfa by regulating antioxidant activity and ion homeostasis
作者：Yin, YanLing;Yang, TianHui;Li, Shuang;Li, Xiaoning;Wang, Wei;Fan, ShuGao;Yang, TianHui
关键词：MeJA; salt tolerance; antioxidant capacity; ion homeostasis; Medicago sativa
-
Genome-Wide Identification and Expression Analysis of the CesA/Csl Gene Superfamily in Alfalfa (Medicago sativa L.)
作者：Sod, Bilig;Xu, Lei;Liu, Yajiao;He, Fei;Xu, Yanchao;Li, Mingna;Kang, Junmei;Yang, Qingchuan;Long, Ruicai;Yang, Tianhui;Gao, Ting
关键词：alfalfa; CesA/Csl; cell wall; forage quality; phylogenetic analysis; gene expression
-
The Efficacy of Biological Control for the Suppression of the Pea Aphid (Acyrthosiphon Pisum): Does the Resistance of Alfalfa Cultivars Matter?
作者：Zhang, Xiang;Wu, Qiong;Mu, Jianing;Chao, Zunqi;He, Qi;Lu, Zhaozhi;Gao, Ting;Wang, Chuan;McNeill, Mark R.;Lu, Zhaozhi
关键词：alfalfa; biological control; pea aphid; conservation biological control
-
Overexpression of the elongation factor MtEF1A1 promotes salt stress tolerance in Arabidopsis thaliana and Medicago truncatula
作者：Xu, Lei;Yang, Qingchuan;Xu, Lei;Zhang, Lixia;Liu, Yajiao;Sod, Bilig;Li, Mingna;Yang, Qingchuan;Long, Ruicai;Yang, Tianhui;Gao, Ting
关键词：Medicago truncatula; Elongation factor; Transgenic; Salt stress; Calmodulin
