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Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System

文献类型： 外文期刊

作者： Wang, Yaping 1 ; Peng, Yanhong 1 ; Liu, Xiaoyan 1 ; Zhou, Ronghua 1 ; Liao, Xianqing 1 ; Min, Yong 1 ; Hu, Yong 3 ; Wang, Ying 1 ; Rao, Ben 1 ;

作者机构： 1.Hubei Acad Agr Sci, Natl Biopesticide Engn Technol Res Ctr, Hubei Innovat Ctr Agr Sci & Technol, Hubei Biopesticide Engn Res Ctr,Biopesticide Bran, Wuhan 430064, Peoples R China

2.Hubei Univ, Engn Hubei Collaborat Innovat Ctr Green Transform, State Key Lab Biocatalysis & Enzyme, Hubei Key Lab Ind Biotechnol,Biol Fac, Wuhan 430062, Peoples R China

3.Hubei Univ Technol, Natl 111 Ctr Cellular Regulat & Mol Pharmaceut, Hubei Res Ctr Food Fermentat Engn & Technol, Minist Educ,Key Lab Fermentat Engn,Hubei Key Lab, Wuhan 430068, Peoples R China

关键词： 2; 3-butanediol; acetoin; Serratia marcescens; whole-cell biocatalysts; NADH; NAD(+) regeneration system

期刊名称：CATALYSTS （ 影响因子：4.501； 五年影响因子：4.641 ）

ISSN：

年卷期： 2021 年 11 卷 12 期

页码：

收录情况： SCI

摘要： An auto-inducing expression system was developed that could express target genes in S. marcescens MG1. Using this system, MG1 was constructed as a whole-cell biocatalyst to produce 2,3-butanediol/acetoin. Formate dehydrogenase (FDH) and 2,3-butanediol dehydrogenase were expressed together to build an NADH regeneration system to transform diacetyl to 2,3-butanediol. After fermentation, the extract of recombinant S. marcescens MG1ABC (pETDuet-bdhA-fdh) showed 2,3-BDH activity of 57.8 U/mg and FDH activity of 0.5 U/mg. And 27.95 g/L of 2,3-BD was achieved with a productivity of 4.66 g/Lh using engineered S. marcescens MG1(Pswnb+pETDuet-bdhA-fdh) after 6 h incubation. Next, to produce 2,3-butanediol from acetoin, NADH oxidase and 2,3-butanediol dehydrogenase from Bacillus subtilis were co-expressed to obtain a NAD+ regeneration system. After fermentation, the recombinant strain S. marcescens MG1ABC (pSWNB+pETDuet-bdhA-yodC) showed AR activity of 212.4 U/mg and NOX activity of 150.1 U/mg. We obtained 44.9 g/L of acetoin with a productivity of 3.74 g/Lh using S. marcescens MG1ABC (pSWNB+pETDuet-bdhA-yodC). This work confirmed that S. marcescens could be designed as a whole-cell biocatalyst for 2,3-butanediol and acetoin production.

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