Development of a Novel Primer-TaqMan Probe Set for Diagnosis and Quantification of Meloidogyne enterolobii in Soil Using qPCR and Droplet Digital PCR Assays
文献类型： 外文期刊
作者： Chen, Yuan 1 ; Long, Haibo 1 ; Feng, Tuizi 1 ; Pei, Yueling 1 ; Sun, Yanfang 1 ; Zhang, Xinchun 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Haikou 571101, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Hainan Key Lab Monitoring & Control Trop Agr Pest, Haikou 571101, Hainan, Peoples R China
关键词： Meloidogyne enterolobii; TaqMan probe; qPCR; ddPCR; identification; quantification
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 19 期
页码：
收录情况： SCI
摘要： Early detection of pathogens before the planting season is valuable to forecast disease occurrence. Therefore, rapid and reliable diagnostic approaches are urgently needed, especially for one of the most aggressive root knot nematodes, Meloidogyne enterolobii. In this study, we developed a novel primer-TaqMan probe set aimed at M. enterolobii. The primer-probe set was successfully applied in the identification and quantification of M. enterolobii via qPCR technology. It was also suitable for improved PCR technology, known as ddPCR analyses, and this work presents the first application of this technology for plant parasitic nematodes. Compared with qPCR, ddPCR exhibited better performance with regard to analytical sensitivity, which can provide a more accurate detection of M. enterolobii concealed in field soil. In addition, we generated standard curves to calculate the number of eggs in soil using the qPCR and ddPCR platforms. Hopefully, the results herein will be helpful for forecasting disease severity of M. enterolobii infection and adopting effective management strategies.
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