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Paenibacillus polymyxa Antagonism towards Fusarium: Identification and Optimisation of Antibiotic Production

文献类型： 外文期刊

作者： Ran, Junjian 1 ; Wu, Youzhi 2 ; Zhang, Bo 1 ; Su, Yiwei 1 ; Lu, Ninghai 1 ; Li, Yongchao 1 ; Liang, Xinhong 1 ; Zhou, Haixu 1 ; Shi, Jianrong 3 ;

作者机构： 1.Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Peoples R China

2.Shanghai Zhongqiao Vocat & Technol Univ, Sch Food & Drug, Shanghai 201514, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Qual & Safety, Nanjing 210014, Peoples R China

关键词： Paenibacillus polymyxa; antibiotic; deoxynivalenol; response surface methodology

期刊名称：TOXINS （ 2022影响因子：4.2； 五年影响因子：4.7 ）

ISSN：

年卷期： 2023 年 15 卷 2 期

页码：

收录情况： SCI

摘要： An antibiotic produced by Paenibacillus polymyxa 7F1 was studied. The 7F1 strain was isolated from the rhizosphere of a wheat field. Response surface methodology was used to optimize the physicochemical parameters. The strain showed broad-spectrum activity against several plant pathogens. Identification of the strain was realized based on 16s rRNA gene and gyrB gene sequencing. The antibiotic was optimized by one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The suitable antibiotic production conditions were optimized using the one-factor-at-a-time method. The individual and interaction effects of three independent variables: culture temperature, initial pH, and culture time, were optimized by Box-Behnken design. The 16SrRNA gene sequence (1239 nucleotides) and gyrB gene (1111 nucleotides) were determined for strain 7F1 and shared the highest identities to those of Paenibacillus polymyxa. The results showed the optimal fermentation conditions for antibiotics produced by Paenibacillus polymyxa 7F1 were a culture temperature of 38 degrees C, initial pH of 8.0, and culture time of 8 h. The antibiotics produced by Paenibacillus polymyxa 7F1 include lipopeptides such as iturin A and surfactin. The results provide a theoretical basis for the development of bacteriostatic biological agents and the control of mycotoxins.

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