Characterization of a broad-spectrum endolysin LysSP1 encoded by a Salmonella bacteriophage
文献类型： 外文期刊
作者： Jiang, Yanhua 1 ; Xu, Dongqin 1 ; Wang, Lianzhu 1 ; Qu, Meng 1 ; Li, Fengling 1 ; Tan, Zhijun 1 ; Yao, Lin 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Minist Agr & Rural Affairs, 106 Nanjing Rd, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol, Qingdao 266033, Peoples R China
关键词： Bacteriophage; Endolysin; Salmonella; Bactericidal activity
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）
ISSN： 0175-7598
年卷期： 2021 年 105 卷 13 期
页码：
收录情况： SCI
摘要： Foodborne pathogens have caused many public health incidents and heavy economic burden. Endolysins have been proven to have efficient bactericidal activity against pathogens with low incidence of resistance. In this study, the recombinant endolysin LysSP1 encoded by Salmonella Typhimurium lytic bacteriophage SLMP1 was obtained by prokaryotic expression, and its characteristics were analyzed. Ethylenediaminetetraacetic acid (EDTA) can be used as the outer membrane permeabilizer to increase the bactericidal activity of LysSP1. Under the synergism of 5 mmol/L EDTA, LysSP1 exhibited a strong bactericidal activity against Salmonella Typhimurium ATCC14028. LysSP1 was stable at 4 degrees C for 7 days and at -20 degrees C for 180 days. LysSP1 remained the optimal activity at 40 degrees C and was efficiently active at alkaline condition (pH 8.0-10.0). Divalent metal ions could not enhance the bactericidal activity of LysSP1 and even caused the significant reduction of bactericidal activity. LysSP1 not only could lyse Salmonella, but also could lyse other Gram-negative strains and Gram-positive strains. These results indicated that LysSP1 is a broad-spectrum endolysin and has potential as an antimicrobial agent against Salmonella and other foodborne pathogens.
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