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Genome-wide identification of Cellulose-like synthase D gene family in Dendrobium catenatum

文献类型： 外文期刊

作者： Xi, Hangxian 1 ; Liu, Jingjing 1 ; Li, Qing 2 ; Chen, Xueliang 1 ; Liu, Chen 1 ; Zhao, Yuxue 1 ; Yao, Jinbo 1 ; Chen, Don 1 ;

作者机构： 1.Zhejiang A&F Univ, SFGA Engn Res Ctr Dendrobium Catenatum D Officina, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China

2.Naval Med Univ, Sch Pharm, Dept Pharmaceut Bot, Shanghai, Peoples R China

3.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Biotech Res Inst, Shanghai 201106, Peoples R China

关键词： Dendrobium catenatum; Cellulose synthase-like D; gene family; polysaccharide biosynthesis; expression patterns

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2021 年 35 卷 1 期

页码：

收录情况： SCI

摘要： Dendrobium catenatum, which grows on the semi humid rocks in the mountains, has been at the top of the Nine Immortals of China since ancient times. It is a kind of yin tonic medicine and its main active component is polysaccharide. Cellulose synthase-like D(CslD) genes were predicted to catalyse the biosynthesis of 1,4-beta-d-glycan backbone of hemicelluloses, which plays fundamental roles in plant development. To investigate the role of CslD in the development of D. catenatum, eight CslD genes (DcCslD1,2a,2b,3a,3b,4a,4b,5) were identified. The results of protein prediction and analysis showed that CslD2a/2b/4a/4b proteins were acidic proteins. All the proteins had obvious hydrophobic or hydrophilic regions, and had transmembrane structure. Phylogenetic analysis revealed that the DcCslD family could be divided into group I, II, III and IV. DcCslD proteins had a typical Cellulose synthase domain and similar protein structures to the CslDs of other plants. Their promoter regions contain cis regulatory elements related to stress and hormone response. The results of qRT-PCR showed that the identified DcCslDs were differentially expressed in roots, stems and leaves. Most of them were highly expressed in stems and leaves. The environmental stresses examination showed that the expression levels of DcCslD5 were closely associated with drought-recovery treatment; the expression levels of DcCslD1, DcCslD2a, DcCslD2b, DcCslD3a and DcCslD5 were significantly influenced by low temperature. This study systematically analyzed the sequence characteristics of CslD protein of D. catenatum, which can provide reference for further study on the function of CslD protein in the polysaccharide metabolism of D. catenatum.

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