Development of a Recombinase Polymerase Amplification Method Combined with a Lateral Flow Dipstick Assay for Rapid Detection of the Larch Pathogen Neofusicoccum laricinum
文献类型： 外文期刊
作者： Ju, Fangyi 1 ; Qi, Zhongqiang 2 ; Tan, Jiajin 1 ; Liu, Tingli 3 ; Dai, Tingting 1 ;
作者机构： 1.Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China
3.Nanjing Xiaozhuang Univ, Sch Food Sci, Nanjing 211171, Peoples R China
4.Nanjing Forestry Univ, Adv Anal & Testing Ctr, Nanjing 210037, Jiangsu, Peoples R China
关键词：
lateral flow dipstick;
期刊名称：PLANT DISEASE （ 影响因子：4.4； 五年影响因子：4.8 ）
ISSN： 0191-2917
年卷期： 2025 年 109 卷 2 期
页码：
收录情况： SCI
摘要： Neofusicoccum laricinum, an important pathogenic species, causes shoot blight of larch. In China, large areas of Larix principis-rupprechtii forests are threatened by this pathogen. Currently, this pathogen is on the list of quarantine pests in China. Because of the widespread and severe damage caused by N. laricinum, a reliable and accurate diagnostic tool is urgently needed. In this study, we first identified Nlar12009 as a N. laricinum-specific gene through genomic sequence data and bioinformatic analysis. Specific primer pairs and DNA probes were designed to detect the target pathogen using a novel recombinase polymerase amplification assay with a lateral flow dipstick (RPA-LFD) method. We optimized the RPA-LFD assay to ensure high specificity to N. laricinum. Our results showed that the assay exclusively detected N. laricinum isolates with no cross-reaction with other isolates of fungal and oomycete species and nematodes. Furthermore, our detection technique exhibited a 10-fold higher sensitivity (10 fg/ml) than conventional polymerase chain reaction for N. laricinum detection. Our developed RPA-LFD assay is proved to be a highly specific, sensitive, time-saving, and convenient method for the diagnosis of N. laricinum and shows great potential in field application.
- 相关文献
作者其他论文 更多>>
-
Comparative transcriptome profiling reveals the key genes and molecular mechanisms involved in rice under blast infection
作者：Li, Gang;Wang, Jian;Cheng, Baoshan;Wang, Di;Gao, Hao;Xu, Weijun;Wang, Wei;Gao, Qingsong;Zhang, Wenxia;Ji, Jianhui;Li, Bianhao;Zhang, Guoliang;Qi, Zhongqiang;Liu, Yongfeng
关键词：Rice; Blast; Transcriptome; Disease resistance; Hormones; Biochemical indicators
-
Rapid and Visual Detection of Pyricularia oryzae Using Coupled Recombinase Polymerase Amplification-Lateral Flow Dipstick Assay
作者：Du, Yan;Qi, Zhongqiang;Yu, Junjie;Zhang, Rongsheng;Yu, Mina;Cao, Huijuan;Song, Tianqiao;Pan, Xiayan;Wang, Shuchen;Liu, Yongfeng;Yang, Jun;Xiong, Qin;Liu, Yongfeng
关键词：disease diagnosis; lateral flow dipstick;
Pyricularia oryzae ; recombinase polymerase amplification -
Woronin body protein UvHex1 is essential for pathogenesis, and contributes to conidiation, cell stress response, lipid metabolism, and mycotoxins production in rice false smut fungus Ustilaginoidea virens
作者：Mao, Yijun;Deng, Qingchao;Yu, Junjie;Mao, Yijun;Pan, Xiayan;Yu, Mina;Cao, Huijuan;Song, Tianqiao;Qi, Zhongqiang;Du, Yan;Zhang, Rongsheng;Liu, Yongfeng;Yu, Junjie
关键词：
Ustilaginoidea virens ; Woronin body; UvHex1; Pathogenicity -
Rhizosphere microbes facilitate the break of chlamydospore dormancy and root colonization of rice false smut fungi
作者：Pan, Xiayan;Zhao, Fengjuan;Song, Tianqiao;Li, Zhi;Qi, Zhongqiang;Yu, Junjie;Cao, Huijuan;Yu, Mina;Liu, Yongfeng;Yue, Yang;Xu, Boting;Shen, Qirong;Xiong, Wu;Li, Zhi;Liu, Yongfeng;Xu, Jinrong
关键词：
-
UvPomt, an O-Methyltransferase Interacting with UvMAT1-1-3, for Regulating Growth, Stress Tolerance, and Virulence in Ustilaginoidea virens
作者：Li, Zhi;Liu, Yongfeng;Li, Zhi;Yu, Junjie;Yu, Mina;Cao, Huijuan;Song, Tianqiao;Wang, Shuchen;Qi, Zhongqiang;Du, Yan;Pan, Xiayan;Liu, Yongfeng
关键词：rice false smut;
Ustilaginoidea virens ; fungal pathogenicity; fungi sexual reproduction; mating-type gene; O-methyltransferase -
Two aquaporins, PIP1;1 and PIP2;1, mediate the uptake of neonicotinoid pesticides in plants
作者：Wan, Qun;Cheng, Jinjin;Wang, Ya;Ge, Jing;Ma, Liya;Li, Yong;Sun, Xing;Chen, Xiaolong;Yu, Xiangyang;Wan, Qun;Li, Yixin;Cheng, Jinjin;Wang, Ya;Ge, Jing;Ma, Liya;Li, Yong;Liu, Jianan;Zhou, Chunli;Li, Haocong;Sun, Xing;Chen, Xiaolong;Yu, Xiangyang;Liu, Tingli;Li, Qing X.
关键词：neonicotinoid; aquaporin; Brassica rapa; plasma membrane; uptake; systemic pesticide
-
The Ras GTPase-activating protein UvGap1 orchestrates conidiogenesis and pathogenesis in the rice false smut fungus Ustilaginoidea virens
作者：Cao, Huijuan;Gong, Hao;Yu, Mina;Pan, Xiayan;Song, Tianqiao;Yu, Junjie;Qi, Zhongqiang;Du, Yan;Zhang, Rongsheng;Liu, Yongfeng;Liu, Yongfeng
关键词：conidiation; pathogenicity; Ras GTPase-activating protein; Ustilaginoidea virens
