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The Transcription Factor MbWRKY46 in Malus baccata (L.) Borkh Mediate Cold and Drought Stress Responses

文献类型： 外文期刊

作者： Liu, Wanda 1 ; Wang, Tianhe 1 ; Wang, Yu 1 ; Liang, Xiaoqi 2 ; Han, Jilong 1 ; Hou, Ruining 1 ; Han, Deguo 2 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Hort Branch, Harbin 150040, Peoples R China

2.Northeast Agr Univ, Coll Hort & Landscape Architecture, Natl Local Joint Engn Res Ctr Dev & Utilizat Small, Key Lab Biol & Genet Improvement Hort Crops Northe, Harbin 150030, Peoples R China

关键词： M; baccata (L; ) Borkh; MbWRKY46; cold stress; drought stress; transgenic plant

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 15 期

页码：

收录情况： SCI

摘要： The living environment of plants is not static; as such, they will inevitably be threatened by various external factors for their growth and development. In order to ensure the healthy growth of plants, in addition to artificial interference, the most important and effective method is to rely on the role of transcription factors in the regulatory network of plant responses to abiotic stress. This study conducted bioinformatics analysis on the MbWRKY46 gene, which was obtained through gene cloning technology from Malus baccata (L.) Borkh, and found that the MbWRKY46 gene had a total length of 1068 bp and encodes 355 amino acids. The theoretical molecular weight (MW) of the MbWRKY46 protein was 39.76 kDa, the theoretical isoelectric point (pI) was 5.55, and the average hydrophilicity coefficient was -0.824. The subcellular localization results showed that it was located in the nucleus. After conducting stress resistance studies on it, it was found that the expression of MbWRKY46 was tissue specific, with the highest expression level in roots and old leaves. Low temperature and drought had a stronger induction effect on the expression of this gene. Under low temperature and drought treatment, the expression levels of several downstream genes related to low temperature and drought stress (AtKIN1, AtRD29A, AtCOR47A, AtDREB2A, AtERD10, AtRD29B) increased more significantly in transgenic Arabidopsis. This indicated that MbWRKY46 gene can be induced to upregulate expression in Arabidopsis under cold and water deficient environments. The results of this study have a certain reference value for the application of M. baccata MbWRKY46 in low-temperature and drought response, and provide a theoretical basis for further research on its function in the future.

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