High-quality wild barley genome assemblies and annotation with Nanopore long reads and Hi-C sequencing data
文献类型： 外文期刊
作者： Pan, Rui 1 ; Hu, Haifei 2 ; Xiao, Yuhui 7 ; Xu, Le 1 ; Xu, Yanhao 1 ; Ouyang, Kai 7 ; Li, Chengdao 2 ; He, Tianhua 2 ; Zhang, Wenying 1 ;
作者机构： 1.Yangtze Univ, Res Ctr Crop Stresses Resistance Technol, Jingzhou 434025, Peoples R China
2.Murdoch Univ, Coll Sci Hlth Engn & Educ, Western Australian State Agr Biotechnol Ctr, Western Crop Genet Alliance, Murdoch, WA 6155, Australia
3.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou 510640, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Genet & Breeding High Qual Rice Southern C, Guangzhou 510640, Peoples R China
5.Guangdong Key Lab New Technol Rice Breeding, Guangzhou 510640, Peoples R China
6.Guangdong Rice Engn Lab, Guangzhou 510640, Peoples R China
7.Grand Biotechnol Co Ltd, Wuhan 430076, Peoples R China
8.Yangtze Univ, Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434025, Peoples R China
9.Dept Primary Ind & Reg Dev, South Perth, WA 6155, Australia
10.Yangtze Univ, MARA Key Lab Sustainable Crop Prod Middle Reaches, Jingzhou 434025, Peoples R China
期刊名称：SCIENTIFIC DATA （ 影响因子：9.8； 五年影响因子：10.8 ）
ISSN：
年卷期： 2023 年 10 卷 1 期
页码：
收录情况： SCI
摘要： Wild barley, from "Evolution Canyon (EC)" in Mount Carmel, Israel, are ideal models for cereal chromosome evolution studies. Here, the wild barley EC_S1 is from the south slope with higher daily temperatures and drought, while EC_N1 is from the north slope with a cooler climate and higher relative humidity, which results in a differentiated selection due to contrasting environments. We assembled a 5.03 Gb genome with contig N50 of 3.53 Mb for wild barley EC_S1 and a 5.05 Gb genome with contig N50 of 3.45 Mb for EC_N1 using 145 Gb and 160.0 Gb Illumina sequencing data, 295.6 Gb and 285.35 Gb Nanopore sequencing data and 555.1 Gb and 514.5 Gb Hi-C sequencing data, respectively. BUSCOs and CEGMA evaluation suggested highly complete assemblies. Using full-length transcriptome data, we predicted 39,179 and 38,373 high-confidence genes in EC_S1 and EC_N1, in which 93.6% and 95.2% were functionally annotated, respectively. We annotated repetitive elements and non-coding RNAs. These two wild barley genome assemblies will provide a rich gene pool for domesticated barley.
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