文献类型： 外文期刊

作者： Miao, Xiaomeng ¹ ; Liu, Jia ³ ; Gong, Qian ³ ; Li, Fugui ⁴ ; Zhang, Yalan ¹ ; Liang, Qiyue ¹ ; Li, Diyan ⁵ ; Ning, Zhonghua ¹ ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding, Beijing 100193, Peoples R China

2.Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang 550005, Peoples R China

3.Guizhou Prov Dept Agr & Rural Affairs, Guizhou Prov Livestock & Poultry Genet Resources M, Guiyang 550001, Peoples R China

4.Tarim Univ, Coll Life Sci & Technol, Xinjiang Prod & Construct Corps, Key Lab Protect & Utilizat Biol Resources Tarim Ba, Alar 843300, Peoples R China

5.Chengdu Univ, Sch Pharm, Chengdu 610106, Peoples R China

关键词： eggshell quality; uterus; chicken; transcriptome; metabolome

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 16 卷 4 期

页码：

收录情况： SCI

摘要： Background/Objectives: Eggshell quality is a critical factor influencing consumer preference and the economic benefits of poultry enterprises, and the uterus is the key site for eggshell synthesis. Yaoshan chicken (YS), an indigenous chicken breed in China, is renowned for its flavorful meat and high-quality eggs. However, its egg production is lower compared to specialized strains. Therefore, the GYR crossbreed was developed by three-line hybridization for YS chicken, which can produce green-shelled eggs with better eggshell thickness and strength than YS chicken (p < 0.01). To explore the molecular mechanisms underlying the differences in eggshell quality between GYR and YS chickens, we conducted an integrated transcriptomic and metabolomic analysis. Methods: Twelve uterus samples (six from GYR and six from YS chickens) were collected during the period of eggshell calcification at 260 days of age. RNA sequencing (RNA-seq) and liquid chromatography-mass spectrometry (LC-MS/MS) were performed to identify differentially expressed genes (DEGs) and differential metabolites (DMs), respectively. Results: A total of 877 DEGs were identified in the GYR group, including 196 upregulated and 681 downregulated genes (|log(2) (fold change)| > 1, p-value < 0.05). Additionally, 79 DMs were detected, comprising 50 upregulated and 29 downregulated metabolites (|log(2) (fold change)| > 1, VIP > 1). Notably, the key DEGs (SLCO1B3, SLCO1B1, PTGR1, LGR6, MELTF, CRISP2, GVINP1, and OVSTL), important DMs (prostaglandin-related DMs and biliverdin) and signaling pathways (calcium signaling, neuroactive ligand-receptor interaction, arachidonic acid metabolism, bile secretion, and primary bile acid biosynthesis) were major regulators of the eggshell quality. Furthermore, an integrated transcriptomic and metabolomic analysis revealed two significant gene-metabolite pairs associated with eggshell quality: PTGDS-prostaglandin E2 and PTGS1-prostaglandin E2. Conclusions: This study provides a theoretical foundation for the improved eggshell quality of Yaoshan chicken.