Differential Expression of Duplicate Insulin-like Growth Factor-1 Receptors (igf1rs) in Medaka Gonads
文献类型： 外文期刊
作者： Wei, Wenbo 1 ; Zhu, Yefei 1 ; Yuan, Cancan 1 ; Zhao, Yuli 1 ; Zhou, Wenzong 2 ; Li, Mingyou 1 ;
作者机构： 1.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
2.Shanghai Acad Agr Sci, Ecoenvironm Protect Res Inst, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
3.Yeasen Biotechnol Co Ltd, 800 Qingdai Rd, Shanghai 201318, Peoples R China
4.Shanghai Xihua Sci Co Ltd, Bldg 6-118,Furonghua Rd, Shanghai 201318, Peoples R China
关键词： igf1rs; gene expression; medaka; ovary; testis
期刊名称：LIFE-BASEL （ 影响因子：3.251； ）
ISSN：
年卷期： 2022 年 12 卷 6 期
页码：
收录情况： SCI
摘要： Insulin-like growth factor-1 receptors (igf1rs) play important roles in regulating development, differentiation, and proliferation in diverse organisms. In the present study, subtypes of medaka igf1r, igf1ra, and igf1rb were isolated and characterized. RT-PCR results showed that igf1ra and igf1rb mRNA were expressed in all tissues and throughout embryogenesis. Using real-time PCR, the differential expression of igf1ra and igf1rb mRNA during folliculogenesis was observed. The results of in situ hybridization (ISH) revealed that both of them were expressed in ovarian follicles at different stages, and igf1rb was also expressed in theca cells and granulosa cells. In the testis, both igf1ra and igf1rb mRNA were highly expressed in sperm, while igf1rb mRNA was also obviously detected in spermatogonia. In addition, igf1ra mRNA was also present in Leydig cells in contrast to the distribution of igf1rb mRNA in Sertoli cells. Collectively, we demonstrated that differential igf1rs RNA expression identifies medaka meiotic germ cells and somatic cells of both sexes. These findings highlight the importance of the igf system in the development of fish gonads.
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