Development and characterization of 16 polymorphic microsatellite loci in the Tibet endemic fish Glyptosternum maculatum
文献类型: 外文期刊
作者: Guo, B. Y. 1 ; Xie, C. X. 1 ; Shan, L. 1 ; Zhang, Y. 2 ; Wang, H. L. 1 ; Xiong, D. M. 1 ; Liu, H. P. 1 ; Ji, Q. 1 ;
作者机构: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Fish Germplasm Resources & Bio, Chinese Minist Agr, Jinzhou 434000, Hubei, Peoples R China
关键词: Glyptosternum maculatum;microsatellite;population genetics
期刊名称:MOLECULAR ECOLOGY RESOURCES ( 影响因子:7.09; 五年影响因子:8.745 )
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收录情况: SCI
摘要: Sixteen novel polymorphic microsatellite loci were developed and characterized in Glyptosternum maculatum, an endemic fish inhabiting the Yarlung Zangbo River valley, Tibet, China. These loci were developed after the enrichment of microsatellite-containing genomic DNA fragments with a subtractive hybridization protocol. The polymorphism of these loci was analysed with 36 individuals representing a geographical population. The number of alleles found at these loci ranged from 3 to 10. The observed heterozygosity and expected heterozygosity ranged from 0 to 1 and from 0.31 to 0.75, respectively. These microsatellite loci will certainly facilitate the determination of the genetic structure of G. maculatum and the conservation of its genetic resource.
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