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Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (Cucumis melo L.)

文献类型： 外文期刊

作者： Han, Hongwei 1 ; Zhou, Yuan 3 ; Liu, Huifang 2 ; Chen, Xianjun 1 ; Wang, Qiang 2 ; Zhuang, Hongmei 2 ; Sun, Xiaoxia 1 ; Ling, Qihua 3 ; Zhang, Huijun 4 ; Wang, Baike 2 ; Wang, Juan 2 ; Tang, Yaping 2 ; Wang, Hao 2 ; Liu, Huiying 1 ;

作者机构： 1.Shihezi Univ, Coll Agr, Dept Hort, Key Lab Special Fruits & Vegetables Cultivat Physi, Shihezi 832003, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Hort Crops, Key Lab Hort Crop Genom & Genet Improvement Xinjia, Urumqi 830002, Peoples R China

3.Chinese Acad Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 200030, Peoples R China

4.Huaibei Normal Univ, Sch Life Sci, Huaibei 235000, Peoples R China

关键词： Hami melon mutant; chloroplast; photosynthesis; photosynthetic carbon assimilation; ROS; transcriptome; metabolism

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 8 期

页码：

收录情况： SCI

摘要： Leaf color mutants are ideal materials for studying the regulatory mechanism of chloroplast development and photosynthesis. We isolated a cucumis melo spontaneous mutant (MT), which showed yellow-green leaf phenotype in the whole growing period and could be inherited stably. We compared its leaves with the wild type (WT) in terms of cytology, physiology, transcriptome and metabolism. The results showed that the thylakoid grana lamellae of MT were loosely arranged and fewer in number than WT. Physiological experiments also showed that MT had less chlorophyll content and more accumulation of reactive oxygen species (ROS) than WT. Furthermore, the activity of several key enzymes in C-4 photosynthetic carbon assimilation pathway was more enhanced in MT than WT. Transcriptomic and metabolomic analyses showed that differential expression genes and differentially accumulated metabolites in MT were mainly co-enriched in the pathways related to photosystem-antenna proteins, central carbon metabolism, glutathione metabolism, phenylpropanoid biosynthesis and flavonoid metabolism. We also analyzed several key proteins in photosynthesis and chloroplast transport by Western blot. In summary, the results may provide a new insight into the understanding of how plants respond to the impaired photosynthesis by regulating chloroplast development and photosynthetic carbon assimilation pathways.

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