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CHARACTERIZATION OF THE GENETIC DIVERSITY OF EPIMEDIUM BREVICORNUM (BERBERIDACEAE) VIA ISSR AND CDDP MARKERS

文献类型： 外文期刊

作者： Duan, Yuanyuan 1 ; Zhou, Wuxian 1 ; You, Jingmao 1 ; Guo, Xiaoliang 1 ; Luo, Xiaorong 1 ; Lu, Chao 1 ; Guo, Jie 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi, Peoples R China

2.Chinese Herbal Med Subctr Hubei Agr Sci & Technol, Enshi, Peoples R China

关键词： Epimedium brevicornum; ISSR; CDDP; Resistance germplasm classification

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）

ISSN： 0556-3321

年卷期： 2021 年 53 卷 4 期

页码：

收录情况： SCI

摘要： Epimedium brevicornum is a traditional and edible medicinal herb, used to tonify kidneys and strengthen tendons and bones. In this study, two types of molecular markers, namely, inter-simple sequence repeat (ISSR) and conserved DNAderived polymorphism (CDDP) markers, were used to evaluate the genetic diversity of E. brevicornum. The results showed that both ISSR and CDDP markers were useful for estimating the genetic diversity. Forty-six polymorphic (86.79%) bands were generated by 7 random ISSR primers, while 82 polymorphic (95.35%) bands were generated by 11 CDDP primers. The genetic similarity coefficients of the ISSR markers ranged from 0.019 to 0.60, while those of the CDDP markers varied from 0.048 to 0.871. Moreover, the CDDP molecular marker technique was a preferable method for marker-assisted selection of stress-resistant varieties. Two superior resistant germplasms (BQ2 and MPC2) were screened, which would enable the selection and breeding of stress-resistant varieties of E. brevicornum.

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