Using a quantum dot bead-based lateral flow immunoassay to broadly detect the adulteration of PDE-5 inhibitors in functional foods
文献类型： 外文期刊
作者： Song, Mingshu 1 ; Wu, Qin 2 ; Liu, Beibei 2 ; Li, Pan 2 ; Jiang, Lan 2 ; Wang, Yulong 2 ; Dong, Sa 2 ; Xiong, Yonghua 1 ; Hammock, Bruce D. 3 ; Zhang, Cunzheng 1 ;
作者机构： 1.Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base Minist Sci & Technol, 50 Zhongling St, Nanjing 210014, Peoples R China
3.Univ Calif Davis, Dept Entomol, Nematol, Davis, CA 95616 USA
4.Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA
5.Jiangsu Univ, Sch Biol & Food Engn, Zhenjiang 212000, Jiangsu, Peoples R China
期刊名称：ANALYTICAL METHODS （ 影响因子：3.532； 五年影响因子：3.244 ）
ISSN： 1759-9660
年卷期： 2022 年 14 卷 26 期
页码：
收录情况： SCI
摘要： In this study, a designed hapten possessing the classic structure of PDE-5 inhibitors was synthesized. A monoclonal antibody (mAb) with broad recognition for six PDE-5 inhibitors was further produced. For the determination of lodenafil, methisosildenafil, mirodenafil, udenafil and tadalafil, the limit of detection (LOD) and IC50 ranged from 1.01 to 26.91 ng mL(-1) and 12.75 to 278 ng mL(-1), respectively. Thereafter, a quantum dot bead-based lateral flow immunoassay (QB-LFIA) was developed, which improved the LOD and IC50 to 0.32-6.52 ng mL(-1) and 7.45-133.8 ng mL(-1), respectively. Method validation was conducted using honey and capsule samples spiked with PDE-5 inhibitors, and the recoveries of the intra- and inter-assays ranged from 81.01% to 108.16%, with coefficients of variation below 12.71%. In addition, the validity and the consistency have been confirmed with a comparison between QB-LFIA and HPLC-MS/MS (R-2 = 0.9957). Furthermore, the developed QB-LFIA was employed for the inspection of real products, and several samples were found to be adulterated with lodenafil and methisosildenafil.
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