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Identification of linear B-cell epitopes of Senecavirus A VP2 protein using monoclonal antibodies

文献类型： 外文期刊

作者： Jiang, Yao 1 ; Guo, Zhenhua 2 ; Weng, Maoyang 2 ; Chen, Linlin 2 ; Li, Qingmei 2 ; Zhang, Lei 3 ; Qiao, Songlin 2 ; Zhang, Gaiping 1 ;

作者机构： 1.Northwest A&F Univ, Coll Vet Med, Xianyang, Shaanxi, Peoples R China

2.Henan Acad Agr Sci, Inst Anim Hlth, Key Lab Anim Immunol Minist Agr, Zhengzhou, Peoples R China

3.China Anim Husb Ind Co Ltd, Rural Affairs Key Lab Vet Biol Prod & Chem, Minist Agr, Beijing, Peoples R China

4.Peking Univ, Sch Adv Agr Sci, Beijing, Peoples R China

5.Longhu Lab, Zhengzhou, Henan, Peoples R China

关键词： Senecavirus A; VP2 protein; monoclonal antibodies; B-cell epitopes; spatial structure

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

ISSN：

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Introduction Senecavirus A (SVA) is an emerging vesicular pathogen in swine with clinical signs similar to those of foot-and-mouth disease, porcine vesicular disease and vesicular stomatitis, making the control of swine vesicular disease outbreaks difficult. Therefore, the development of effective diagnostics and vaccines for SVA has become critical. VP2 is a structural protein that elicits a strong immune response, which positions it a candidate for diagnostic and vaccine development.Methods In this study, five high-titer monoclonal antibodies (mAbs) were produced using hybridoma technology. Twenty-eight peptides covering the entire VP2 sequence were synthesised by overlapping peptide synthesis, and the positive peptides were screened with the five mAbs by ELISA and Dot-blotting. The peptides were then further truncated to identify the minimal epitope regions based on immunoinformatics analyses.Results Four mAbs were identified that reacted with peptide 15 and one mAb reacted with peptide 26. Further truncation of these peptides led to the identification of two novel minimal epitopes: 156-NEEQWV-161 and 262-VRPTSPYFN-270. Structural and sequence alignment analyses revealed that epitope 156-NEEQWV-161 is located in the flex-loop region of the VP2, whereas epitope 262-VRPTSPYFN-270 is located in the beta-sheet of the VP2. Both epitopes were highly conserved among typical SVA isolates from different countries.Discussion This study identifies two novel B-cell epitopes on the VP2, contributing to the development of VP2-based diagnostic tools with clinical applications. The findings also provide valuable material for the design of novel vaccines against SVA, offering new insights into the immune response to this pathogen.

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