文献类型： 外文期刊

作者： Bai, Guohui ¹ ; Chen, Bin ¹ ; Xiao, Xin ³ ; Li, Yuexin ¹ ; Liu, Xia ¹ ; Zhao, Dan ⁴ ; Zhang, Lei ⁶ ; Zhao, Degang ⁴ ;

作者机构： 1.Zunyi Med Univ, Hosp Stomatol, Dept Oral Med, Zunyi 563000, Guizhou, Peoples R China

2.Zunyi Med Univ, Sch Stomatol, Key Lab Oral Dis Res, Zunyi 563000, Guizhou, Peoples R China

3.Zunyi Med Univ, Inst Life Sci, Engn Technol Res Ctr Phys & Chem Anal, Zunyi 563000, Guizhou, Peoples R China

4.Guizhou Univ, Key Lab Plant Resources Conservat & Germplasm Inno, Minist Educ, Inst Agrobioengn, Guiyang 550025, Guizhou, Peoples R China

5.Guizhou Univ, Coll Life Sci, Guiyang 550025, Guizhou, Peoples R China

6.Zunyi Med Univ, Sch Pharm, Key Lab Biocatalysis & Chiral Drug Synthesis Guizh, Zunyi 563000, Guizhou, Peoples R China

7.Zunyi Medical Univ, Sch Pharm, Zunyi, Guizhou, Peoples R China

8.Guizhou Acad Agr Sci, Inst Guizhou Distinctt Plant Resources Conservat, Guiyang 550006, Guizhou, Peoples R China

9.Zunyi Med Univ, Sch Pharm, Key Lab Biocatalysis & Chiral Drug Synth Guizhou P, 6 West Xuefu Rd, Zunyi 563000, Guizhou, Peoples R China

10.Guizhou Univ, Key Lab Plant Resources Conservat & Germplasm Inno, Inst Agrobioengn, Minist Educ, Huaxi Ave South Sect 2708, Guiyang 550025, Guizhou, Peoples R China

11.Guizhou Univ, Coll Life Sci, Huaxi Ave South Sect 2708, Guiyang 550025, Guizhou, Peoples R China

关键词： geniposide; iridoid; oral squamous cell carcinoma; anticancer; cell proliferation

期刊名称：EXPERIMENTAL AND THERAPEUTIC MEDICINE （ 影响因子：2.751； 五年影响因子：2.475 ）

ISSN： 1792-0981

年卷期： 2022 年 24 卷 6 期

页码：

收录情况： SCI

摘要： Iridoids are a special class of cyclopentanoid monoterpenes, which exhibit a wide range of biological effects. The present study aimed to investigate the potential effects of three iridoids genipin, geniposide and geniposidic acid on three human oral squamous cell carcinoma (OSCC) cell lines HSC-2, SCC-9 and A253 in addition to studying the possible underlying mechanisms. Cell viability assay revealed that geniposide treatment significantly suppressed the proliferation of all three cancer cell lines. In addition, geniposide induced SCC-9 cell cycle arrest at the G(2)/M phase (flow cytometry) through downregulation of cyclin-dependent kinase 2 and Cyclin A2 expression (western blot analysis), whilst also inducing cell apoptosis (flow cytometry and acridine orange/ethidium bromide staining) by dissipating the mitochondrial membrane potential (flow cytometry), and upregulating the expression of cleaved caspase-3 and cleaved poly-ADP ribose polymerase (western blot analysis). A wound-healing assay indicated that geniposide impaired SCC-9 cell migration by increasing the expression of E-cadherin (western blot analysis), whilst suppressing the expression of MMP-2 (western blot analysis). Western blot analysis also demonstrated that geniposide induced autophagy in SCC-9 cells by upregulating the expression of Beclin-1 and light chain 3-II. Mechanistically, geniposide activated the 5'-AMP-activated protein kinase signaling pathway and inhibited the JNK signaling pathway in SCC-9 cells (western blot analysis). The present results indicated that geniposide is able to inhibit the proliferation and migration of the tongue squamous carcinoma cell line SCC-9, suggesting a potential strategy for OSCC treatment.