Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis
文献类型： 外文期刊
作者： Chen, Shan 1 ; Jiang, Tao 1 ; Lin, Hao 3 ; Chen, Junyan 1 ; Yang, Shuangli 1 ; Wang, Pengcheng 1 ; Gan, Xinqiang 4 ; Wang 1 ;
作者机构： 1.Nanjing Med Univ, Sch Pharm, Key Lab Cardiovasc & Cerebrovasc Med, Nanjing 211166, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Facil & Equipment, Key Lab Protected Agr Engn Middle & Lower Reaches, Minist Agr & Rural Affairs, Nanjing 210014, Jiangsu, Peoples R China
3.Southeast Univ, Zhongda Hosp, Sch Med, Dept Clin Sci & Res, Nanjing 210009, Jiangsu, Peoples R China
4.Southeast Univ, Zhongda Hosp, Sch Med, Dept Urol, Nanjing 210009, Jiangsu, Peoples R China
期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）
ISSN： 0003-2700
年卷期： 2021 年 93 卷 29 期
页码：
收录情况： SCI
摘要： Fast detection of low-concentration exosomes in body fluids is of great significance in understanding the pathogenesis and disease diagnosis but is quite a challenging work due to the complex matrix, tedious pretreatment, and relatively poor sensitivity without the aid of instruments. In this work, by simply using a filter membrane to enrich the exosomes at low concentrations and the use of CuS nanoparticles as labels, we were able to detect exosomes at concentrations as low as 2 x 10(3) particles/mu L in a complex matrix by the naked eye. Due to its high sensitivity, specificity, and simplicity, it can be used for the diagnosis of direct prostate cancer via a 5 mL urine sample within 2 h without the use of any instrument. This method can also be applicable for the detection of other biological nanoparticles, such as viruses, at low concentrations in a complex matrix, offering a promising candidate for point-of-care disease diagnosis with low cost.
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