Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis
文献类型： 外文期刊
作者： Chen, Shan 1 ; Jiang, Tao 1 ; Lin, Hao 3 ; Chen, Junyan 1 ; Yang, Shuangli 1 ; Wang, Pengcheng 1 ; Gan, Xinqiang 4 ; Wang 1 ;
作者机构： 1.Nanjing Med Univ, Sch Pharm, Key Lab Cardiovasc & Cerebrovasc Med, Nanjing 211166, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Facil & Equipment, Key Lab Protected Agr Engn Middle & Lower Reaches, Minist Agr & Rural Affairs, Nanjing 210014, Jiangsu, Peoples R China
3.Southeast Univ, Zhongda Hosp, Sch Med, Dept Clin Sci & Res, Nanjing 210009, Jiangsu, Peoples R China
4.Southeast Univ, Zhongda Hosp, Sch Med, Dept Urol, Nanjing 210009, Jiangsu, Peoples R China
期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）
ISSN： 0003-2700
年卷期： 2021 年 93 卷 29 期
页码：
收录情况： SCI
摘要： Fast detection of low-concentration exosomes in body fluids is of great significance in understanding the pathogenesis and disease diagnosis but is quite a challenging work due to the complex matrix, tedious pretreatment, and relatively poor sensitivity without the aid of instruments. In this work, by simply using a filter membrane to enrich the exosomes at low concentrations and the use of CuS nanoparticles as labels, we were able to detect exosomes at concentrations as low as 2 x 10(3) particles/mu L in a complex matrix by the naked eye. Due to its high sensitivity, specificity, and simplicity, it can be used for the diagnosis of direct prostate cancer via a 5 mL urine sample within 2 h without the use of any instrument. This method can also be applicable for the detection of other biological nanoparticles, such as viruses, at low concentrations in a complex matrix, offering a promising candidate for point-of-care disease diagnosis with low cost.
- 相关文献
作者其他论文 更多>>
-
Production of polyunsaturated fatty acids in pork backfat fermented by Mucor circinelloides
作者：Shi, Haisu;Lin, Hao;Yan, Yiran;Wang, Ruhong;Wu, Rina;Wu, Junrui;Shi, Haisu;Wu, Rina;Wu, Junrui;Shi, Haisu;Wu, Rina;Wu, Junrui;Zhang, Yingtong
关键词：Mucor circinelloides; Ultrasound; Papain; Polyunsaturated fatty acids; Fermented pork backfat
-
Electrochemical Sensor for Ammonium Nitrogen in Soil Based on Platinum Modified Screen-printed Electrodes
作者：Chen, Shan;Liu, Jun;Qian, Mingyan;Chen, Jingwen;Chen, Jinhu;Fang, Yimin;Xu, Guohailin
关键词：Soil; Ammonium nitrogen; Electrochemistry; Screen printed electrode
-
Modification and Application of Bamboo-Based Materials: A Review-Part I: Modification Methods and Mechanisms
作者：Zheng, Zhiyu;Yan, Nina;Jiang, Xizhi;Zhang, Xiaomei;Chen, Shan;Xu, Rui;Liu, Chun;Xu, Lei;Lou, Zhichao
关键词：bamboo; sustainability; modification
-
Modification and Application of Bamboo-Based Materials: A Review-Part II: Application of Bamboo-Based Materials
作者：Lou, Zhichao;Zheng, Zhiyu;Yan, Nina;Jiang, Xizhi;Zhang, Xiaomei;Chen, Shan;Xu, Rui;Liu, Chun;Xu, Lei;Zheng, Zhiyu;Yan, Nina;Jiang, Xizhi;Zhang, Xiaomei;Chen, Shan;Xu, Rui;Liu, Chun;Xu, Lei
关键词：bamboo; sustainability; application
-
Cadmium ions mediated turn-on electrogenerated chemiluminescence of ZnS nanoparticles for highly selective cadmium detection in seafood
作者：Chen, Shan;Chen, Jingwen;Qian, Mingyan;Liu, Jun;Zheng, Yuhao;Sun, Hao;Chen, Jinghu;Fang, Yimin;Zhang, Biqi;Sun, Jian-Jun;Huang, Zongxiong;Xu, Guohailin
关键词：Electrogenerated chemiluminescence; Cation exchange; Cadmium ion; ZnS nanoparticles; Food samples
-
A link between STK signalling and capsular polysaccharide synthesis in Streptococcus suis
作者：Tang, Jinsheng;Guo, Mengru;Chen, Min;Ma, Zhe;Lin, Huixing;Fan, Hongjie;Xu, Bin;Ran, Tingting;Wang, Weiwu;Ma, Zhe;Lin, Huixing;Fan, Hongjie
关键词：
-
Low cost, portable voltammetric sensors for rapid detection of nitrate in soil
作者：Chen, Shan;Qian, Mingyan;Liu, Jun;Chen, Jinghu;Fang, Yimin
关键词：Nitrate; Cadmium sulfide; Screen printed electrodes; On -site detection; Soil samples
