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Occurrence, Characteristics, and PCR-Based Detection of Pectobacterium polaris Causing Soft Rot of Chinese Cabbage in China

文献类型： 外文期刊

作者： Chen, Changlong 1 ; Li, Xiaoying 1 ; Bo, Zijing 1 ; Du, Wenxiao 2 ; Fu, Lu 1 ; Tian, Yu 1 ; Cui, Shuang 1 ; Shi, Yanxia 3 ; Xie, Hua 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing 100097, Peoples R China

2.Univ Yantai, Sch Life Sci, Yantai 264005, Shandong, Peoples R China

3.Chinese Acad Agr Sci, Sch Inst Vegetables & Flowers, Beijing 100081, Peoples R China

关键词： average nucleotide identity; bacterial soft rot; Chinese cabbage (Brassica rapa L. ssp. pekinensis); Pectobacterium polaris; phenotypic characteristics; phylogeny; specific PCR/qPCR detection

期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）

ISSN： 0191-2917

年卷期： 2021 年 105 卷 10 期

页码：

收录情况： SCI

摘要： Bacterial soft rot is an important disease of Chinese cabbage (Brassica rapa L. ssp. pekinensis) in China and many other countries. Four pectinolytic bacterial strains (WBC1, WBC6, WBC9, and WBC11) were isolated from soft-rotted Chinese cabbage in Beijing, China. Based on 16S rDNA and pmrA gene sequence analyses, multilocus sequence analysis (MLSA), and genomic average nucleotide identity (ANI) analysis, these four strains were identified as Pectobacterium polaris. This species, previously reported from potato in countries not including China, is a new soft rot pathogen of Chinese cabbage in China. Biochemical characteristics of these P. polaris strains tested by Biolog were mostly consistent with those of P. polaris NIBIO1006T. Their pathogenicity on Chinese cabbage is temperature dependent, with all four strains as well as the type strain exhibiting high pathogenicity at 23 degrees C and 28 degrees C. These four strains infected Lactuca sativa, Daucus carota, Solanum tuberosum, and Capsicum annuum by artificial inoculation. Specific polymerase chain reaction (PCR) and quantitative PCR (qPCR) primers for P. polaris were developed on the basis of its specific gene sequences (determined by genome comparison methods). Both PCR and qPCR detected not only genomic DNA of P. polaris but also the pathogen from diseased plant tissues even before external symptoms appeared. Their detection sensitivities were as low as 1 pg and 100 pg genomic DNA of P. polaris, respectively. To our knowledge, this study is the first to both report the emergence of P. polaris on Chinese cabbage in China and provide rapid and accurate PCR/ qPCR-based detection systems specific for P. polaris.

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