文献类型： 外文期刊

作者： Su, Yanyan ¹ ; Li, Xiaoying ¹ ; Li, Lei ² ; Lukianova, Anna ³ ; Tokmakova, Anna ³ ; Chen, Changlong ¹ ; Fu, Lu ¹ ; Tian, Yu ¹ ; Shi, Yanxia ² ; Xie, Jianbo ⁴ ; Miroshnikov, Konstantin A. ³ ; Yang, Jungang ¹ ; Xie, Hua ¹ ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

3.Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

4.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, Beijing 100083, Peoples R China

关键词： characterization; Chinese cabbage (Brassica rapa L. subsp. pekinensis); host range; Pectobacterium versatile; qPCR-based detection; soft rot

期刊名称：PLANT DISEASE （ 影响因子：4.5； 五年影响因子：5.0 ）

ISSN： 0191-2917

年卷期： 2023 年 107 卷 9 期

页码：

收录情况： SCI

摘要： Pectobacterium is one of the most important genera of phytopathogenic bacteria. It can cause soft-rot diseases on a wide range of plant species across the world. In this study, three Pectobacterium strains (KC01, KC02, and KC03) were isolated from soft-rotted Chinese cabbage in Beijing, China. These three strains were identified as Pectobacterium versatile based on phylogenetic analysis of Pectobacterium 16S ribosomal RNA, pmrA, and 504 Pectobacterium core genes, as well as a genomic average nucleotide identity analysis. Their biochemical characteristics were found to be similar to the P. versatile type strain ICMP9168(T) but differed in response to citric acid, stachyose, D-glucuronic acid, dextrin, and N-acetyl-beta-D-mannosamine. All of the tested P. versatile strains showed different carbohydrate utilization abilities compared with P. carotovorum and P. odoriferum, particularly in their ability to utilize D-arabitol, L-rhamnose, and L-serine. Under laboratory conditions, the maceration ability of P. versatile on Chinese cabbage was the highest at 28 degrees C, compared with those at 13, 28, 23, and 33 degrees C. Additionally, P. versatile could infect all of the 17 known Pectobacterium host plants, except for Welsh onion (Allium fistulosum). A SYBR Green quantitative PCR (qPCR) detection system was developed to distinguish P. versatile from other soft-rot bacteria based on the combined performance of melting curve (with a single melting peak at around 85 degrees C) and fluorescence curve (with cycle threshold <30) when the bacterial genomic DNA concentration was in the range of 10 pg/mu l to 10 ng/mu l. This study is the first to report the presence of P. versatile on Chinese cabbage in China, as well as a specific and sensitive qPCR assay that can be used to quickly identify P. versatile. The work contributes to a better understanding of P. versatile and will facilitate the effective diagnosis of soft-rot disease, ultimately benefitting commercial crop production.