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Wax Crystal-Sparse Leaf1 encodes a beta-ketoacyl CoA synthase involved in biosynthesis of cuticular waxes on rice leaf

文献类型： 外文期刊

作者： Yu, Dongmei 1 ; Ranathunge, Kosala 3 ; Huang, Huasun 4 ; Pei, Zhongyou 1 ; Franke, Rochus 3 ; Schreiber, Lukas 3 ; He, 1 ;

作者机构： 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Microbiol, Beijing 100101, Peoples R China

2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

3.Univ Bonn, Inst Cellular & Mol Bot, Dept Ecophysiol, D-53115 Bonn, Germany

4.Chinese Acad Trop Agr Sci, Rubber Res Inst, Hainan 571737, Peoples R China

关键词： cuticular waxes;beta-ketoacyl CoA synthase;rice (Oryza sativa L.);T-DNA insertional mutagenesis;Wax Crystal-Sparse Leaf1;FATTY-ACID ELONGASE;ARABIDOPSIS-THALIANA;EPICUTICULAR WAX;CONDENSING ENZYME;SEQUENCE-ANALYSIS;T-DNA;FUNCTIONAL-CHARACTERIZATION;SUBSTRATE-SPECIFICITY;LESQUERELLA-FENDLERI;EPIDERMAL-CELLS

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

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收录情况： SCI

摘要： Cuticular waxes, forming the plant/atmosphere interface of plants colonizing the terrestrial environment, are complex mixtures of very-long chain fatty acids (VLC-FAs) and their derivatives. In VLCFAs biosynthesis, beta-ketoacyl CoA synthase (E.C.2.3.1.119, KCS) is the key enzyme. Using T-DNA insertional mutagenesis, we identified a cuticle-deficient rice mutant, which displayed a pleiotropic phenotype including reduced growth, leaf fusion, sparse wax crystals, enhanced sensitivity to drought and low fertility. Further analysis indicated that T-DNA was inserted in the 5'-UTR intron of the affected gene, Wax Crystal-Sparse Leaf1 (WSL1), and abnormal transcript caused the loss-of-function of WSL1 gene. Genetic complementation experiment confirmed the function of the candidate gene. WSL1 was predicted to encode a polypeptide containing a conserved FAE1_CUT1_RppA domain typical of the KCS family proteins. Qualitative and quantitative wax composition analyses by gas chromatography-mass spectrometry (GC-MS) demonstrated a marked reduction of total cuticular wax load on wsl1 leaf blades and sheaths, and VLCFA precursors of C20-C24 decreased in both. Moreover, ubiquitous expression of the WSL1 gene gave a hint that WSL1-catalyzed elongation of VLCFAs might participate in a wide range of rice growth and development processes beyond biosynthesis of cuticular waxes.

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