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DNA Methylation Correlates with the Expression of Drought-Responsive Genes and Drought Resistance in Rice

文献类型： 外文期刊

作者： Ding, Guohua 1 ; Cao, Liangzi 1 ; Zhou, Jinsong 1 ; Li, Zhugang 1 ; Lai, Yongcai 5 ; Liu, Kai 5 ; Luo, Yu 1 ; Bai, Liangming 1 ; Wang, Xueyang 1 ; Wang, Tongtong 1 ; Wang, Rongsheng 6 ; Yang, Guang 1 ; Sun, Shichen 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Tillage, Harbin 150086, Peoples R China

2.Minist Agr, Key Lab Combining Farming & Anim Husb, Harbin 150086, Peoples R China

3.Heilongjiang Prov Key Lab Crop Mol Design & Germp, Harbin 150086, Peoples R China

4.Heilongjiang Rice Qual Improvement & Genet Breedi, Harbin 150086, Peoples R China

5.Natl Salt Tolerant Rice Technol Innovat Ctr, Northeast Branch, Harbin 150086, Peoples R China

6.Heilongjiang Acad Agr Sci, Biotechnol Res Inst, Harbin 150086, Peoples R China

关键词： rice accessions; delta C-13 values; drought stress; transcriptomes; methylomes

期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）

ISSN：

年卷期： 2022 年 12 卷 6 期

页码：

收录情况： SCI

摘要： The delta C-13 value is regarded as an important indicator for tolerance to drought stress (DS), which is a severe abiotic stress that influences rice productivity. However, exploration of droughtresponsive genes (DRGs) related to delta C-13 regulation is less reported. In this study, we investigated the natural variation in delta C-13 values in 102 japonica rice accessions. Among them, two rice accessions with contrasting delta C-13 values, Longdao 10 (LD10, DS-tolerant) and Binxu (BX, DS-sensitive), were used for further analysis. LD10 possesses better drought resistance with 2% lower 6 13 C values, 35% lower stomatal length and density, 33% lower water loss, and 11% lower stomatal conductance in comparison to BX. Transcriptome analysis shows that there are 2325 and 1378 differentially expressed genes (DEGs) induced by DS in LD10 and BX at the tillering stage, respectively, while there are 1076 and 492 DEGs in LD10 and BX at the graining stage, respectively. In total, 21 overlapped DEGs (defined as DRGs) were identified due to DS effects across two rice accessions over two stages. Among them, the expression levels of six genes, including chloride transporter (CLT1) and photosystem II polypeptide (PSBP), were further tested using qRT-PCR. Furthermore, we found that four methyltransferase genes were upregulated in BX compared to LD10 under DS. Consistently, the methylation levels of CLT1 and PSBP were higher along both promoter and CDS regions for CG, CHG, and CHH types. This study highlights the importance of the expression of these DRGs in response to DS and provides deep insights into DNA methylation-driven gene expression conferring different drought responses in rice.

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