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Characterization of Interactions between the Soybean Salt-Stress Responsive Membrane-Intrinsic Proteins GmPIP1 and GmPIP2

文献类型： 外文期刊

作者： Liu, Jia 1 ; Qi, Weicong 1 ; Lu, Haiying 1 ; Shao, Hongbo 1 ; Zhang, Dayong 5 ;

作者机构： 1.Environm Jiangsu Acad Agr Sci JAAS, Inst Agr Resources, Salt Soil Agr Ctr, Key Lab Agr Environm Lower Reaches Yangtze River, Nanjing 210014, Peoples R China

2.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing 210014, Peoples R China

3.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224002, Peoples R China

4.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao 266000, Peoples R China

5.Nanjing Agr Univ, Hybrid Cotton R&D Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Minist Educ, Nanjing 210095, Peoples R China

关键词： gene expression; GmPIPs; protein interactions; salt stress; soybean

期刊名称：AGRONOMY-BASEL （ 影响因子：3.417； 五年影响因子：3.64 ）

ISSN：

年卷期： 2021 年 11 卷 7 期

页码：

收录情况： SCI

摘要： Salt tolerance is an important trait in soybean cultivation and breeding. Plant responses to salt stress include physiological and biochemical changes that affect the movement of water across the plasma membrane. Plasma membrane intrinsic proteins (PIPs) localize to the plasma membrane and regulate the water and solutes flow. In this study, quantitative real-time PCR and yeast two-hybridization were engaged to analyze the early gene expression profiles and interactions of a set of soybean PIPs (GmPIPs) in response to salt stress. A total of 20 GmPIPs-encoding genes had varied expression profiles after salt stress. Among them, 13 genes exhibited a downregulated expression pattern, including GmPIP1;6, the constitutive overexpression of which could improve soybean salt tolerance, and its close homologs GmPIP1;7 and 1;5. Three genes showed upregulated patterns, including the GmPIP1;6 close homolog GmPIP1;4, when four genes with earlier increased and then decreased expression patterns. GmPIP1;5 and GmPIP1;6 could both physically interact strongly with GmPIP2;2, GmPIP2;4, GmPIP2;6, GmPIP2;8, GmPIP2;9, GmPIP2;11, and GmPIP2;13. Definite interactions between GmPIP1;6 and GmPIP1;7 were detected and GmPIP2;9 performed homo-interaction. The interactions of GmPIP1;5 with GmPIP2;11 and 2;13, GmPIP1;6 with GmPIP2;9, 2;11 and GmPIP2;13, and GmPIP2;9 with itself were strengthened upon salt stress rather than osmotic stress. Taken together, we inferred that GmPIP1 type and GmPIP2 type could associate with each other to synergistically function in the plant cell; a salt-stress environment could promote part of their interactions. This result provided new clues to further understand the soybean PIP-isoform interactions, which lead to potentially functional homo- and heterotetramers for salt tolerance.

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