Rapid detection of strawberry mottle virus using reverse transcription recombinase polymerase amplification with lateral flow strip
文献类型： 外文期刊
作者： Zou, Xiaohua 1 ; Dong, Chao 1 ; Ni, Yiduo 1 ; Yuan, Shidong 1 ; Gao, Qing-Hua 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
关键词： Strawberry; SMoV; RT-RPA; Diagnosis
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.623； 五年影响因子：2.239 ）
ISSN： 0166-0934
年卷期： 2022 年 307 卷
页码：
收录情况： SCI
摘要： Strawberry mottle virus (SMoV) is one of the main RNA viruses that profoundly affects the growth of strawberries worldwide. The rapid on-site detection of SMoV described here can be applied to produce virus-free strawberry seedlings. Reverse transcriptase recombinase polymerase amplification (RT-RPA) was combined with lateral flow (LF) strip to rapidly detect SMoV. The detection limit was 500 fg of RNA under optimized conditions. The SMoV-RT-RPA-LF assay was optimal with a combination of 2 mu L reverse primer (5 mu M) and 0.6 mu L probe (10 mu M) in a 50 mu L RT-RPA reaction mixture for isothermal amplification at 40 degrees C for 15 min. In addition, 100 suspected samples were collected from different regions in the Shanghai suburbs. The SMoV-RT-RPA-LF assay showed that 3 of these 100 samples were positive for SMoV, which was in good concordance with the reverse transcription polymerase chain reaction (RT-PCR) results. The primers and probe had a unique specificity to SMoV because there was no cross-reaction with other strawberry viruses. This study provides an effective technique for the rapid on-site detection of SMoV to ensure a virus-free strawberry nursery.
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