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Golden 2-like transcription factor contributes to the major QTL against rice black-streaked dwarf virus disease

文献类型： 外文期刊

作者： Li, Xuejuan 1 ; Lin, Feng 1 ; Li, Chenyang 1 ; Du, Linlin 1 ; Liu, Zhiyang 5 ; Shi, Wenjuan 1 ; Lv, Jianying 1 ; Cao, Xiaoyan 1 ; Lan, Ying 1 ; Fan, Yongjian 1 ; Zhou, Yijun 1 ; Zhou, Tong 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Safety State Key Lab Cultivat Base, Nanjing 210014, Jiangsu, Peoples R China

3.Int Rice Res Inst, Nanjing 210014, Jiangsu, Peoples R China

4.Jiangsu Acad Agr Sci Joint Lab, Nanjing 210014, Jiangsu, Peoples R China

5.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Jiangsu, Peoples R China

6.Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 12 期

页码：

收录情况： SCI

摘要： Key message A major resistance QTL was identified on chromosome 6 in rice variety Wuke; both overexpression and knockdown experiments confirmed that OsGLK1 is the candidate gene for association with Rice black-streaked dwarf virus disease. Rice black-streaked dwarf virus disease is one of the most destructive rice viral diseases in China and East Asia. Progress has been limited in RBSDVD resistance breeding due to inadequate knowledge on the underlying functional genes. In this study, a major QTL for RBSDV (rice black-streaked dwarf virus) independent of SBPH (small brown planthopper) resistance was mapped in a 1.8 Mb interval on chromosome 6 by using an F-2:3 population originated from resistant rice variety Wuke. Representative transcripts within this region were analysed and three genes showing amino acid sequence variation in functional domains were selected for transformation. Overexpression experiments showed that one gene exhibited significant enhanced resistance compared to control lines, encoding protein involving Myb domain and probable transcription factor Golden 2-like1 (GLK1). Furthermore, OsGLK1 knockdown rice lines were investigated and the resistance ability was significantly declined without this gene compared to the wild type. Taken together, both overexpression and knockdown experiments strongly suggested that OsGLK1 plays an important role for RBSDV resistance and contributes to the major QTL. The study paves the way for elucidating the molecular mechanism underlying RBSDVD resistance and the molecular markers associated with OsGLK1 may be used for marker-assisted selection.

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