您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale

文献类型： 外文期刊

作者： Wu, Shuanghua 1 ; Zhang, Ting 1 ; Wang, Yudan 1 ; Chen, Muxi 1 ; Yang, Jianguo 2 ; Li, Fei 3 ; Deng, Ying 3 ; Zhu, Zhangsheng 1 ; Lei, Jianjun 1 ; Chen, Guoju 1 ; Cao, Bihao 1 ; Chen, Changming 1 ;

作者机构： 1.South China Agr Univ, Coll Hort, Minist Agr & Rural Areas, Key Lab Biol & Germplasm Enhancement Hort Crops So, Guangzhou 510642, Peoples R China

2.Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Peoples R China

3.Guizhou Acad Agr Sci, Inst Hort, Guiyang 550000, Peoples R China

关键词： progoitrin glucosinolate; ODD; biosynthesis of glucosinolates; gene function; Chinese kale

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 23 期

页码：

收录情况： SCI

摘要： Progoitrin (2-hydroxy-3-butenyl glucosinolate, PRO) is the main source of bitterness of Brassica plants. Research on the biosynthesis of PRO glucosinolate can aid the understanding of the nutritional value in Brassica plants. In this study, four ODD genes likely involved in PRO biosynthesis were cloned from Chinese kale. These four genes, designated as BocODD1-4, shared 75-82% similarities with the ODD sequence of Arabidopsis. The sequences of these four BocODDs were analyzed, and BocODD1 and BocODD2 were chosen for further study. The gene BocODD1,2 showed the highest expression levels in the roots, followed by the leaves, flowers, and stems, which is in accordance with the trend of the PRO content in the same tissues. Both the expression levels of BocODD1,2 and the content of PRO were significantly induced by high- and low-temperature treatments. The function of BocODDs involved in PRO biosynthesis was identified. Compared with the wild type, the content of PRO was increased twofold in the over-expressing BocODD1 or BocODD2 plants. Meanwhile, the content of PRO was decreased in the BocODD1 or BocODD2 RNAi lines more than twofold compared to the wildtype plants. These results suggested that BocODD1 and BocODD2 may play important roles in the biosynthesis of PRO glucosinolate in Chinese kale.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台