BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale
文献类型： 外文期刊
作者： Wu, Shuanghua 1 ; Zhang, Ting 1 ; Wang, Yudan 1 ; Chen, Muxi 1 ; Yang, Jianguo 2 ; Li, Fei 3 ; Deng, Ying 3 ; Zhu, Zhangsheng 1 ; Lei, Jianjun 1 ; Chen, Guoju 1 ; Cao, Bihao 1 ; Chen, Changming 1 ;
作者机构： 1.South China Agr Univ, Coll Hort, Minist Agr & Rural Areas, Key Lab Biol & Germplasm Enhancement Hort Crops So, Guangzhou 510642, Peoples R China
2.Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Peoples R China
3.Guizhou Acad Agr Sci, Inst Hort, Guiyang 550000, Peoples R China
关键词： progoitrin glucosinolate; ODD; biosynthesis of glucosinolates; gene function; Chinese kale
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 23 期
页码：
收录情况： SCI
摘要： Progoitrin (2-hydroxy-3-butenyl glucosinolate, PRO) is the main source of bitterness of Brassica plants. Research on the biosynthesis of PRO glucosinolate can aid the understanding of the nutritional value in Brassica plants. In this study, four ODD genes likely involved in PRO biosynthesis were cloned from Chinese kale. These four genes, designated as BocODD1-4, shared 75-82% similarities with the ODD sequence of Arabidopsis. The sequences of these four BocODDs were analyzed, and BocODD1 and BocODD2 were chosen for further study. The gene BocODD1,2 showed the highest expression levels in the roots, followed by the leaves, flowers, and stems, which is in accordance with the trend of the PRO content in the same tissues. Both the expression levels of BocODD1,2 and the content of PRO were significantly induced by high- and low-temperature treatments. The function of BocODDs involved in PRO biosynthesis was identified. Compared with the wild type, the content of PRO was increased twofold in the over-expressing BocODD1 or BocODD2 plants. Meanwhile, the content of PRO was decreased in the BocODD1 or BocODD2 RNAi lines more than twofold compared to the wildtype plants. These results suggested that BocODD1 and BocODD2 may play important roles in the biosynthesis of PRO glucosinolate in Chinese kale.
- 相关文献
作者其他论文 更多>>
-
Map-based cloning revealed BhAPRR2 gene regulating the black peel formation of mature fruit in wax gourd (Benincasa hispida)
作者：Zhai, Xuling;Yan, Jinqiang;Liu, Wenrui;Cao, Zhenqiang;Mo, Renlian;Wang, Baochen;Cheng, Xiaoxin;Xie, Dasen;Jiang, Biao;Li, Zheng;Deng, Ying
关键词：
-
A comprehensive analysis of the O-methyltransferase gene family in the chili pepper (Capsicum annuum) identifies COMT36 involved in capsaicinoids biosynthesis
作者：Li, Shanglin;Cai, Yutong;Yang, Ruyi;Chen, Guoju;Chen, Changming;Zhu, Zhangsheng;Ma, Hongmei;Liu, Yixuan;Zheng, Jie;Zhou, Xin;He, Jianwen;Wu, Di
关键词：Capsicum; O-methyltransferase; Gene family; Capsaicin
-
Comprehensive transcriptome analysis reveals StMAPK7 regulate cold response in potato
作者：Luo, Xiaobo;Ye, Ximiao;Zhao, Degang;Luo, Xiaobo;Chen, Mingjun;Li, Fei;Luo, Xiaobo
关键词：Potato; Cold stress; Cold acclimation; Transcriptome; WGCNA; StMAPK7
-
Impact of different drying methods on the quality and flavor of two chili peppers (Capsicum annuum L.) varieties: Chemical composition and volatile compounds
作者：Wu, Di;Yang, Wanrong;Chen, Ju;Wang, Nanyi;He, Jianwen;Fu, Wenting;Zhu, Zhangsheng;Chen, Changming;Wu, Kangyun
关键词：Pepper; Drying; Volatiles; Chemical components; Correlation analysis
-
Overexpression of StBBX14 Enhances Cold Tolerance in Potato
作者：Zhang, Heng;Song, Li;Zhang, Heng;Chen, Mingjun;Luo, Xiaobo;Li, Fei;Luo, Xiaobo
关键词：potato; cold resistance; BBX gene; overexpression; transcriptome; WGCNA
-
Genome-wide identification and expression analysis of the CONSTANS-like family in potato (Solanum tuberosum L.)
作者：Yin, Wang;Wang, Luo;Shu, Qiqiong;Chen, Mingjun;Li, Fei;Luo, Xiaobo;Wang, Luo;Li, Fei;Luo, Xiaobo;Wang, Luo;Luo, Xiaobo
关键词：CONSTANS-like; gene family; tuberization; cold stress; qRT-PCR
-
Comparative Transcriptomics Reveal the Mechanisms Underlying the Glucosinolate Metabolic Response in Leaf Brassica juncea L. under Cold Stress
作者：Tang, Bing;Zhang, Bao-Hui;Mo, Chuan-Yuan;Fu, Wen-Yuan;Yang, Wei;Wang, Qing-Qing;Ao, Ning;Qu, Fei;Tan, Guo-Fei;Tao, Lian;Deng, Ying
关键词：transcriptomics; Brassica juncea L; glucosinolate; cold stress; transcription factor
