文献类型： 外文期刊
作者： Tao, Ziqian 1 ; Ma, Yanping 2 ; Hao, Le 2 ; Jia, Mengyan 2 ; Huang, Wen 4 ; Liu, Zhenxing 2 ;
作者机构： 1.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China
2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China
3.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China
4.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China
5.GDAAS, Collaborat Innovat Ctr, Guangzhou 510640, Peoples R China
关键词： epigoitrin; mandarin fish ranavirus; interferon-stimulated genes
期刊名称：FISH PATHOLOGY （ 影响因子：0.2； 五年影响因子：0.4 ）
ISSN： 0388-788X
年卷期： 2024 年 59 卷 2 期
页码：
收录情况： SCI
摘要： The mandarin fish ranavirus (MRV), classified within the genus Ranavirus of the Iridoviridae family, is a lethal pathogen to Mandarin fish ( Siniperca chuatsi). ). Currently, no approved treatment exists, underscoring the urgent need for therapeutic strategies. In this study, we aimed to investigate the antiviral activity of epigoitrin against MRV. An in vitro antiviral activity assay revealed that the CC50 of epigoitrin is 43.16 mu M , as determined by the CCK-8 assay. Epigoitrin effectively reduced the number of MRV copies from 8.63 x 10(8) copies/mL to 1.82 x 10(8) copies/mL, exhibiting dose-dependent inhibition of MRV replication. The effective concentration (EC50) of epigoitrin was further determined to be 13.72 mu M . Moreover, epigoitrin diminished the MRV-induced cytopathic effect as observed under a light microscope and suppressed the expression of major caspid protein of MRV (MRV-MCP), as visualized by immunofluorescence assay. It was revealed that epigoitrin primarily exerts its antiviral effects during the stage of viral replication. Further investigation into the immune response revealed that epigoitrin significantly upregulated the gene expression of Mx1 (5-fold), IFITM (2.5-fold), viperin (10- fold), ISG15 (15-fold), and IRF5 (2-fold). This suggests that epigoitrin indirectly unleashes its antiviral function by activating the host's interferon (IFN) immune response. In conclusion, our findings indicate that epigoitrin holds great potential as a therapeutic agent against MRV infection by modulating the IFN pathway.
- 相关文献
作者其他论文 更多>>
-
Mechanisms of immune responses in Acanthopagrus latus to Streptococcus agalactiae infection revealed by transcriptomic analysis
作者：Fan, Hong-Di;Zhao, Yuan-Ming;Lin, Jia;Wang, Jiang-Hai;Liu, Min;Wang, Xu;Huang, Wen
关键词：Acanthopagrus latus; Streptococcus agalactiae; Transcriptomic analysis; Differentially expressed genes; Immune responses; Phagosome-lysosomal pathway
-
Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides)
作者：Zhou, Donglai;Zhong, Wenhao;Fu, Bing;Li, Erna;Li, Qingrong;Yang, Qiong;Zou, Yuxiao;Liao, Sentai;Xing, Dongxu;Zhou, Donglai;Li, Erna;Wang, Fubao;Zhong, Wenhao;Hao, Le;Liu, Zhenxing
关键词：largemouth bass; growth; serum biochemistry; liver metabolism; inflammation
-
Identification of novel strain Acinetobacter baumannii H1 and its improvement capacity for nutrient removal after coimmobilized on activated carbon and CaCO3 in real aquaculture wastewater
作者：Ma, Yonghao;Shu, Hu;Zhou, Jiayi;Liu, Yuting;Lai, Xunheng;Lin, Junduo;Yue, Sha;Ma, Yonghao;Lu, Huijie;Zhao, Jichen;Liu, Fengkun;Guo, Qiang;Huang, Wen;Ma, Yonghao
关键词：Bacterial immobilization; Aerobic nitrogen and phosphate removal; Wastewater treatment; Bacterial community
-
Lycorine provides antiviral activities against mandarin fish ranavirus via suppressing apoptosis and activating type I interferon pathway
作者：Jia, Mengyan;Guo, Hui;Jia, Mengyan;Ma, Yanping;Hao, Le;Tao, Ziqian;Wang, Jingya;Liu, Zhenxing;Tao, Ziqian;Wang, Jingya;Liao, Sentai
关键词：MRV; Lycorine; Apoptosis; Interferon; ROS
-
Mandarin Fish Ranavirus (MRV) Infection Induced Inflammation and Histologic Lesions in the Gut of Mandarin Fish
作者：Gao, Xinru;Zhang, Ziye;Li, Yugu;Gao, Xinru;Zhang, Ziye;Ma, Yanping;Hao, Le;Liu, Zhenxing;Gao, Xinru;Zhang, Ziye;Ma, Yanping;Hao, Le;Liu, Zhenxing;Huang, Wen
关键词：histologic lesions; inflammation; mandarin fish ranavirus; pathogenesis
-
Removal of Nitrogen and Phosphorus by a Novel Salt-Tolerant Strain Pseudomonas sediminis D4
作者：Yin, Peng;Huang, Wen;Liu, Yuting;Zhou, Jiayi;Ma, Yonghao;Lai, Xunheng;Lin, Junduo;Peng, Huilin;Shu, Hu;Ma, Yonghao
关键词：brackish water; simultaneous nitrogen and phosphorus removal; heterotrophic nitrification-aerobic denitrification;
Pseudomonas sediminis ; salt tolerance -
In vitro and in vivo evaluation of the effects of condensed tannins and catechins monomers on antioxidant and intestinal health of Chinese seabass (Lateolabrax maculatus)
作者：Dong, Ruiqi;Qiu, Jianqiang;Cao, Junming;Huang, Wen;Chen, Bing;Zhao, Hongxia;Sun, Wenhao;Lu, Huijie;Peng, Kai;Dong, Ruiqi;Qiu, Jianqiang;Sun, Wenhao;Loh, Jiun-Yan
关键词：condensed tannins; catechins monomers; Lateolabrax maculatus; antioxidant; intestinal health
