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The lipoxygenase OsLOX10 affects seed longevity and resistance to saline-alkaline stress during rice seedlings

文献类型： 外文期刊

作者： Wang, Fuxiang 1 ; Xu, Huibin 2 ; Zhang, Ling 1 ; Shi, Yunrui 2 ; Song, Yu 1 ; Wang, Xinyue 1 ; Cai, Qiuhua 2 ; He, Wei 2 ; Xie, Huaan 1 ; Zhang, Jianfu 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Agr, Fuzhou 350002, Peoples R China

2.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350018, Peoples R China

3.Minist Agr & Affairs, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou 350003, Peoples R China

4.Minist Agr & Affairs, Natl Rice Improvement Ctr China, Fuzhou Branch, Fuzhou 350003, Peoples R China

5.Minist Agr & Affairs, Fujian Engn Lab Crop Mol Breeding, Fuzhou 350003, Peoples R China

6.Minist Sci & Technol, Minist Agr & Affairs, Key Lab Germplasm Innovat & Mol Breeding Hybrid Ri, Incubator Natl Key Lab Germplasm Innovat & Mol Bre, Fuzhou 350003, Peoples R China

7.Minist Agr & Affairs, Fujian Key Lab Rice Mol Breeding, Fuzhou 350003, Peoples R China

关键词： Lipoxygenase OsLOX10; Seed longevity; Free fatty acid; Saline-alkaline stress

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：5.1； 五年影响因子：5.3 ）

ISSN： 0167-4412

年卷期： 2023 年 111 卷 4-5 期

页码：

收录情况： SCI

摘要： Prolonged storage of rice seeds can lead to a decrease in seed vigor and seedling quality. The Lipoxygenase (LOX) gene family is widely distributed in plants, and LOX activity is closely related to seed viability and stress tolerance. In this study, the lipoxygenase OsLOX10 gene from the 9-lipoxygenase metabolic pathway was cloned from rice, and its roles in determining seed longevity and tolerance to saline-alkaline stress caused by Na2CO3 in rice seedlings were mainly investigated. CRISPR/Cas9 knockout of OsLOX10 increased seed longevity compared with the wild-type and OsLOX10 overexpression lines in response to artificial aging. The expression levels of other 9-lipoxygenase metabolic pathway related genes, such as LOX1, LOX2 and LOX3, were increased in the LOX10 overexpression lines. Quantitative real-time PCR and histochemical staining analysis showed that the expression of LOX10 was highest in seed hulls, anthers and the early germinating seeds. KI-I-2 staining of starch showed that LOX10 could catalyze the degradation of linoleic acid. Furthermore, we found that the transgenic lines overexpressing LOX10 showed better tolerance to saline-alkaline stress than the wild-type and knockout mutant lines. Overall, our study demonstrated that the knockout LOX10 mutant increased seed longevity, whereas overexpression of LOX10 enhanced tolerance to saline-alkaline stress in rice seedlings. Key messageThe Lipoxygenase OsLOX10 gene was cloned from rice (Oryza sativa L.). Mutation in LOX10 caused increasing of seed longevity, whereas overexpression of LOX10 enhanced tolerance to saline-alkaline stress in rice seedlings.

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