您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and isolation of a novel antifreeze peptide from crayfish shells

文献类型： 外文期刊

作者： Liu, Mingli 1 ; Bai, Shunjie 1 ; Jiang, Ziwei 1 ; Li, Hailan 1 ; Tu, Ziyi 4 ; Liao, Tao 1 ; Yu, Wei 1 ; Qiu, Liang 1 ;

作者机构： 1.Hubei Acad Agr Sci, Key Lab Cold Chain Logist Technol Agroprod, Minist Agr & Rural Affairs, Inst Agroprod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China

2.Hubei Innovat Ctr Agr Sci & Technol, Agroprod Proc Res Sub Ctr, Wuhan, Peoples R China

3.Wuhan Inst Technol, Sch Chem & Environm Engn, Wuhan, Peoples R China

4.HuBei Crawfish Ind Tech Ltd, Qianjiang 433100, Peoples R China

关键词： Crayfish shells; Antifreeze peptides; Thermal hysteresis activity; Ice crystal growth inhibition; Molecular dynamics simulation

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2024 年 198 卷

页码：

收录情况： SCI

摘要： In this study, a novel antifreeze peptide was isolated from crayfish shells and its antifreeze mechanism and activity were investigated. Firstly, the crayfish shells peptides (CSPs) were prepared by Enzymatic Hydrolysis Technology (alkaline protease and trypsin) and the CSPs could significantly enhance the survival rate of Saccharomyces cerevisiae from 12.5% to 88.5% after 24h freeze-thaw cycle. Then, a total 1004 peptides were identified from CSPs by liquid chromatography/tandem mass spectrometry (LC-MS/MS), among those peptides, two peptide sequences YWDHPIRDGFAPH (AFP1) and GPPGKPGIPDIVDW (AFP1) were selected as potential antifreeze polypeptides (AFPs) according the physicochemical properties: instability index, hydrophilicity, and confidence level. Furthermore, the antifreeze activity and mechanism of AFP1 and AFP2 were studied through molecular dynamics simulation, revealing that the AFP1 and AFP2 could adsorb to ice surface with 7 and 14 hydrogen bonds, respectively. Moreover, AFP2 has a more stable binding capacity and could interact with 33 water molecules. At last, the results of differential scanning calorimetry (DSC) and cold-stage polarization microscope indicated that the thermal hysteresis (TH) values of AFP2 were 1.62 - 2.09 degrees C and could inhibit ice recrystallization. Therefore, this study provides a theoretical basis for developing safe peptide-based cryoprotectants which will improve the quality of frozen foods.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台