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Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China

文献类型： 外文期刊

作者： Gu, Xiaoxiao 1 ; Ma, Xue 1 ; Wu, Qin 1 ; Tao, Qiaoxiaoci 1 ; Chai, Yingjin 1 ; Zhou, Xia 1 ; Han, Mengli 2 ; Li, Jie 1 ; Huang, Xin 2 ; Wu, Tongzhong 2 ; Zhang, Xingxing 2 ; Zhong, Fagang 2 ; Cao, Yiheng 1 ; Zhang, Liyuan 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832000, Xinjiang, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Xinjiang, Peoples R China

关键词： antibiotic resistance; cattle; Escherichia coli infections; multiplex polymerase chain reaction; phylogeny; virulence factors; sheep

期刊名称：VETERINARY MEDICINE AND SCIENCE （ 影响因子：1.7； 五年影响因子：1.8 ）

ISSN：

年卷期： 2023 年

页码：

收录情况： SCI

摘要： BackgroundEscherichia coli infections are common in Xinjiang, a major region of cattle and sheep breeding in China. Therefore, strategies are required to control E. coli. The aim of this study was to investigate the phylogenetic groups, virulence genes, and antibiotic resistance characteristics of E. coli isolates. MethodsIn this study, 116 tissue samples were collected from the organs of cattle and sheep that were suspected of having E. coli infections between 2015 and 2019. Bacteria in the samples were identified using a biochemical identification system and amplification of 16S rRNA, and the phylogenetic groupings of E. coli isolates were determined by multiplex polymerase chain reactions. In addition, PCR detection and analysis of virulence factors, antibiotic resistance genes, and drug-resistant phenotypes of E. coli isolates were performed. ResultsA total of 116 pathogenic E. coli strains belonging to seven phylogenetic groups were isolated, with the majority of isolates in groups A and B1. Among the virulence genes, curli-encoding crl had the highest detection rate of 97.4%, followed by hemolysin-encoding hlyE with the detection rate of 94.82%. Antimicrobial susceptibility test results indicated that the isolates had the highest rates of resistance against streptomycin (81.9%). ConclusionThese characteristics complicate the prevention and treatment of E. coli-related diseases in Xinjiang.

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