您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transcriptome sequencing and DEG analysis in different developmental stages of floral buds induced by potassium chlorate in Dimocarpus longan

文献类型： 外文期刊

作者： Huang, Shilian 1 ; Qiao, Yanchun 4 ; Lv, Xinmin 1 ; Li, Jianguang 1 ; Han, Dongmei 1 ; Guo, Dongliang 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou, Guangdong, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou, Guangdong, Peoples R China

3.Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou, Guangdong, Peoples R China

4.Guangzhou Acad Agr Sci, Guangzhou, Guangdong, Peoples R China

关键词： floral induction; flowering pathway; longan ( Dimocarpus longan ); potassium chlorate; transcriptome

期刊名称：PLANT BIOTECHNOLOGY （ 影响因子：1.308； 五年影响因子：1.621 ）

ISSN： 1342-4580

年卷期： 2022 年 39 卷 3 期

页码：

收录情况： SCI

摘要： Potassium chlorate can promote off-season flowering in longan, but the molecular mechanisms are poorly understood. In this study, four-year-old 'Shixia' longan trees were injected in the trunk with potassium chlorate, and terminal buds were sampled and analyzed using transcriptomics and bioinformatics tools. To generate a reference longan transcriptome, we obtained 207,734 paired-end reads covering a total of 58,514,149 bp, which we assembled into 114,445 unigenes. Using this resource, we identified 3,265 differentially expressed genes (DEGs) that were regulated in longan terminal buds in response to potassium chlorate treatment for 2, 6 or 30 days, including 179 transcription factor genes. By reference to the Arabidopsis literature, we then defined 38 longan genes involved in flowering, from which we constructed the longan flowering pathway. According to RNA-seq data, at least 24 of these genes, which participate in multiple signaling pathways, are involved in potassium chlorate-stimulated floral induction, and the differential regulation in terminal buds of ten floral pathway genes (GI, CO, GID1, GA4, GA5, FLC, AP1, LFY, FT and SOC1) was confirmed by qRT-PCR. These data will contribute to an improved understanding of the functions of key genes involved in longan floral induction by potassium chlorate.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台