文献类型： 外文期刊

作者： Akram, Waheed ¹ ; Waqar, Sara ¹ ; Hanif, Sana ¹ ; Anjum, Tehmina ¹ ; Aftab, Zill-e-Huma ¹ ; Li, Guihua ² ; Ali, Basharat ³ ; Rizwana, Humaira ⁴ ; Hassan, Ali ¹ ; Rehman, Areeba ⁵ ; Munir, Bareera ⁵ ; Umer, Muhammad ¹ ;

作者机构： 1.Univ Punjab, Fac Agr Sci, Dept Plant Pathol, Lahore 54590, Pakistan

2.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Peoples R China

3.Univ Punjab, Inst Microbiol & Mol Genet, Lahore 54590, Pakistan

4.King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11495, Saudi Arabia

5.Univ Punjab, Coll Earth & Environm Sci, Lahore 54590, Pakistan

关键词： tomato; Fusarium wilt; plant growth-promoting bacteria; Bacillus; seed coating; biopriming

期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Beneficial plant microbes can enhance the growth and quality of field crops. However, the benefits of microbes using cheap and efficient inoculation methods are still uncommon. Seed coating with biocontrol agents can reduce the amount of inocula along with having the potential for large-scale application. Hence, in this research work, the comparative potential of tomato seed coating and biopriming with Bacillus aryabhattai Z-48, harboring multiple plant-beneficial traits, to suppress Fusarium wilt disease along with its beneficial effect on seedling and plant growth promotion was analyzed. Among two bacterial strains, B. aryabhattai Z-48 was able to antagonize the mycelial growth of Fusarium oxysporum f.sp. lycopersici in vitro and its application as a seed coating superiorly benefited seedling traits like the germination percentage, vigor index, and seedling growth index along with a reduced germination time. The seed coating with B. aryabhattai Z-48 resulted in significant increases in the shoot length, root length, dry biomass, and total chlorophyll contents when compared with the bioprimed seeds with the same bacterial strain and non-inoculated control plants. The seed coating with B. aryabhattai Z-48 significantly reduced the disease index (>60%) compared with the pathogen control during pot trials. Additionally, the seed coating with B. aryabhattai Z-48 resulted in a significantly higher production of total phenolics, peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase enzyme in tomato plants. The GC/MS-based non-targeted metabolic profiling indicated that the seed coating with B. aryabhattai Z-48 could cause large-scale metabolite perturbations in sugars, sugar alcohols, amino acids, and organic acids to increase the fitness of tomato plants against biotic stress. Our study indicates that a tomato seed coating with B. aryabhattai Z-48 can improve tomato growth and suppress Fusarium wilt disease effectively under conventional agricultural systems.