文献类型： 外文期刊

作者： Xu, Linan ¹ ; Wu, Yajing ³ ; Yang, Xiangpeng ¹ ; Pang, Xinxin ⁴ ; Wu, Yansha ⁵ ; Li, Xingshuai ¹ ; Liu, Xiayu ³ ; Zhao, Yuzhong ¹ ; Yu, Lumin ¹ ; Wang, Peikun ¹ ; Ye, Bin ¹ ; Jiang, Shijin ² ; Ma, Junfei ¹ ; Zhang, Xinglin ¹ ;

作者机构： 1.Linyi Univ, Coll Agr & Forestry, Linyi 276005, Peoples R China

2.Shandong Agr Univ, Coll Vet Med, Dept Prevent Vet Med, Tai An, Peoples R China

3.Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou, Peoples R China

4.Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China

5.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai, Peoples R China

关键词： Enterococcus faecium; Fe-S cluster biosynthesis; anaerobic conditions; gastrointestinal (GI) tract colonization

期刊名称：GUT MICROBES （ 影响因子：12.2； 五年影响因子：12.4 ）

ISSN： 1949-0976

年卷期： 2024 年 16 卷 1 期

页码：

收录情况： SCI

摘要： The facultative anaerobic Gram-positive bacterium Enterococcus faecium is a ubiquitous member of the human gut microbiota. However, it has gradually evolved into a pathogenic and multidrug resistant lineage that causes nosocomial infections. The establishment of high-level intestinal colonization by enterococci represents a critical step of infection. The majority of current research on Enterococcus has been conducted under aerobic conditions, while limited attention has been given to its physiological characteristics in anaerobic environments, which reflects its natural colonization niche in the gut. In this study, a high-density transposon mutant library containing 26,620 distinct insertion sites was constructed. Tn-seq analysis identified six genes that significantly contribute to growth under anaerobic conditions. Under anaerobic conditions, deletion of sufB (encoding Fe-S cluster assembly protein B) results in more extensive and significant impairments on carbohydrate metabolism compared to aerobic conditions. Consistently, the pathways involved in this utilization-restricted carbohydrates were mostly expressed at significantly lower levels in mutant compared to wild-type under anaerobic conditions. Moreover, deletion of sufB or pflA (encoding pyruvate formate lyase-activating protein A) led to failure of gastrointestinal colonization in mice. These findings contribute to our understanding of the mechanisms by which E. faecium maintains proliferation under anaerobic conditions and establishes colonization in the gut.