Exploring the relationship between environmental DNA concentration and biomass in Asian giant softshell turtle (Pelochelys cantorii)
文献类型： 外文期刊
作者： Hong, Xiaoyou 1 ; Wang, Kaikuo 1 ; Ji, Liqin 1 ; Liu, Xiaoli 1 ; Yu, Lingyun 1 ; Wei, Jie 1 ; Wang, Yakun 1 ; Wei, Chengqing 1 ; Li, Wei 1 ; Zhu, Xinping 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr & Rural Affairs, Guangzhou, Peoples R China
2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai, Peoples R China
关键词： Environmental DNA; Pelochelys cantorii; ND5; Quantitative PCR; Biomass
期刊名称：PEERJ （ 影响因子：2.7； 五年影响因子：3.1 ）
ISSN： 2167-8359
年卷期： 2023 年 11 卷
页码：
收录情况： SCI
摘要： In recent years, environmental DNA (eDNA) technology has become an accepted approach for investigating rare and endangered species because of its economic efficiency, high sensitivity, and non-invasiveness. The Asian giant softshell turtle (Pelochelys cantorii) is a first-class protected aquatic animal in China, and traditional resource survey methods have not identified its natural populations for many years. In this study, primers and a TaqMan probe targeting ND5 were designed, reaction conditions were optimized, a standard curve was constructed using synthetic DNA, and an eDNA quantitative PCR (qPCR) detection method was established. The eDNA detection technology for P. cantorii revealed that the number of species in the experimental pools showed a significant linear relationship with the eDNA concentration (p < 0.05). The eDNA concentration was negatively correlated with the length of time after the removal of P. cantorii and retention in the water body for 9 days. The qPCR detection method for P. cantorii eDNA established in this study can be applied to the qualitative detection of P. cantorii in water bodies, as well as to preliminary evaluation of its relative biomass. This can serve as a baseline for the investigation of natural P. cantorii population and the evaluation of its wild release effects.
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