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Genome-Wide Identification and Expression Analysis of the Aquaporin Gene Family in Lycium barbarum during Fruit Ripening and Seedling Response to Heat Stress

文献类型： 外文期刊

作者： He, Wei 1 ; Liu, Mingyu 1 ; Qin, Xiaoya 2 ; Liang, Aihua 3 ; Chen, Yan 1 ; Yin, Yue 2 ; Qin, Ken 2 ; Mu, Zixin 1 ;

作者机构： 1.Northwest A&F Univ, Coll Life Sci, Xianyang 712100, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Peoples R China

3.Tarim Univ, Coll Life Sci & Technol, Alar 843301, Peoples R China

4.State Key Lab Breeding Base Protect & Utilizat Bio, Alaer 843300, Peoples R China

5.Minist Sci & Technol, Alaer 843300, Peoples R China

关键词： Lycium barbarum; aquaporins; genome-wide; water relations; fruit ripening; heat stress

期刊名称：CURRENT ISSUES IN MOLECULAR BIOLOGY （ 影响因子：2.976； 五年影响因子：3.139 ）

ISSN： 1467-3037

年卷期： 2022 年 44 卷 12 期

页码：

收录情况： SCI

摘要： Plant-water relations mediated by aquaporins (AQPs) play vital roles in both key plant growth processes and responses to environmental challenges. As a well-known medicinal and edible plant, the harsh natural growth habitat endows Lycium plants with ideal materials for stress biology research. However, the details of their molecular switch for water transport remain unclear. In the present work, we first identified and characterized AQP family genes from Lycium (L.) barbarum at the genome scale and conducted systemic bioinformatics and expression analyses. The results showed that there were 38 Lycium barbarum AQPs (LbAQPs) in L. barbarum, which were classified into four subfamilies, including 17 LbPIP, 9 LbTIP, 10 LbNIP, and 2 LbXIP. Their encoded genes were unevenly distributed on all 12 chromosomes, except chromosome 10. Three of these genes encoded truncated proteins and three genes underwent clear gene duplication events. Cis-acting element analysis indicated that the expression of LbAQPs may be mainly regulated by biotic/abiotic stress, phytohormones and light. The qRT-PCR assay indicated that this family of genes presented a clear tissue-specific expression pattern, in which most of the genes had maximal transcript levels in roots, stems, and leaves, while there were relatively lower levels in flowers and fruits. Most of the LbAQP genes were downregulated during L. barbarum fruit ripening and presented a negative correlation with the fruit relative water content (RWC). Most of their transcripts presented a quick and sharp upregulation response to heat stress following exposure of the 2-month-old seedlings to a 42 degrees C temperature for 0, 1, 3, 12, or 24 h. Our results proposed that LbAQPs were involved in L. barbarum key development events and abiotic stress responses, which may lay a foundation for further studying the molecular mechanism of the water relationship of Lycium plants, especially in harsh environments.

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