Evaluation of Tissue Tropism and Horizontal Transmission of a Duck Enteritis Virus Vectored Vaccine in One-Day-Old Chicken
文献类型： 外文期刊
作者： Abdulrahim, Yassin 1 ; You, Yingying 2 ; Wang, Linggou 1 ; Bi, Zhixiang 2 ; Xie, Lihua 2 ; Chen, Saisai 2 ; Kaufer, Benedikt B. 6 ; Damiani, Armando Mario 2 ; Huang, Kehe 1 ; Wang, Jichun 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China
3.Nyala Univ, Coll Vet Sci, POB 155, South Darfur, Sudan
4.Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China
5.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Peoples R China
6.Free Univ Berlin, Inst Virol, Robert von Ostertag Str 7-13, D-14163 Berlin, Germany
7.Free Univ Berlin, Vet Ctr Resistance Res TZR, D-14163 Berlin, Germany
8.Univ Nacl Cuyo, Fac Ciencias Med, Lab Bioquim & Inmunidad, RA-5502 Mendoza, Argentina
9.Consejo Nacl Invest Cient & Tecn, IMBECU, RA-5500 Mendoza, Argentina
关键词： vector vaccines; DEV-H5; HVT-VP2; one-day-old chickens
期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）
ISSN：
年卷期： 2024 年 16 卷 11 期
页码：
收录情况： SCI
摘要： Herpesvirus of turkey (HVT) recombinant vector vaccines are widely used in the poultry industry. However, due to limitations in loading multiple foreign antigens into a single HVT vector, other viral vectors are urgently needed. Since chickens lack maternal immunity to duck enteritis virus (DEV), vector vaccines using DEV as a backbone are currently under study. Even though a recently developed DEV vector vaccine expressing the influenza hemagglutinin H5 of highly pathogenic avian influenza (DEV-H5) induces highly detectable anti-HA antibodies, safety issues hamper further vaccine development. In this work, tissue affinity and horizontal transmission in 1-day-old chickens were systematically evaluated after DEV-H5 vector vaccine inoculation. Sixty percent of DEV-H5-inoculated chickens died between day 2 and day 7 post-inoculation. The displayed clinical signs consisted of lethargy, anorexia, and diarrhea, and virus was shed in feces. Gross and/or histological lesions were recorded in the kidney, heart, intestine, liver, lung, and spleen. Moreover, DEV-H5 replication in intestinal cells caused an increment in interferon-alpha expression, while occluding junction proteins and ZO-1 expression were significantly upregulated. As a control, birds inoculated with a commercial recombinant turkey herpesvirus expressing the VP2 protein of the infectious bursal disease virus (HVT-VP2) vector vaccine showed neither clinical signs nor mortality. Overall, while the HVT-VP2 vaccine demonstrated complete safety in 1-day-old chickens, our potential DEV-H5 vaccine requires further attenuation for consideration as a vector vaccine candidate in chickens.
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