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Enzymatic Preparation and Structure-activity Relationship of Sesaminol

文献类型： 外文期刊

作者： Gao, Jinhong 1 ; Wang, Ruidan 1 ; Lu, Xin 1 ; Jia, Cong 2 ; Sun, Qiang 2 ; Huang, Jinian 1 ; Wei, Songli 2 ; Ma, Lin 1 ;

作者机构： 1.Henan Acad Agr Sci, Res Ctr Agr & Sideline Prod Proc, 116 Pk Rd, Zhengzhou 450002, Henan, Peoples R China

2.Henan Acad Agr Sci, Inst Agr & Sideline Prod Proc, 116 Pk Rd, Zhengzhou 450002, Henan, Peoples R China

关键词： sesaminol triglycoside; sesaminol; enzymatic hydrolysis; antioxidant activity; structure-activity relationship

期刊名称：JOURNAL OF OLEO SCIENCE （ 影响因子：1.601； 五年影响因子：1.768 ）

ISSN： 1345-8957

年卷期： 2021 年 70 卷 9 期

页码：

收录情况： SCI

摘要： As a valuable natural antioxidant, sesaminol can be used in food and medicine industries, but it is trace in sesame seeds and oil, and it is feasible to prepare sesaminol from sesaminol triglucoside (STG) which is abundant in defatted sesame cake. Therefore, in order to establish an effective enzymatic preparation method and elucidate the antioxidant structure-activity relationship of sesaminol, a suitable glycosidase for preparing sesaminol from STG were screened, enzymatic hydrolysis was optimized by single-factor test and response surface methodology, and finally, the structure-activity relationship of sesaminol was illustrated by comparative molecular field analysis (CoMFA). These results suggested that beta-galactosidase was the optimal glycosidase for enzymatic hydrolysis of STG to prepare sesaminol. Under the optimal conditions of a reaction temperature of 50 degrees C, reaction time of 4.0 h, pH of 5.5, substrate concentration of 1.0 mg/mL, and enzyme dosage of 20 mg/mL, the conversion rate of sesaminol was 98.88 +/- 0.67%. Sesaminol displayed excellent antioxidant ability in 2,2-diphenyl-1-picrylhydrazyl (DPPH, IC50 = 0.0011 mg/mL), 2,2'-azinobis-(3-ethyl-benzothiazoline-6-sulfonate) (ABTS, IC50 = 0.0021 mg/mL) radical scavenging activities and Ferric reducing antioxidant power (FRAP, 103.2998 mol/g) compared to other sesaminol derivatives. According to -log (IC50 of DPPH) and -log (IC50 of ABTS), CoMFA models were successfully established based on Q(2) >0.5 (Q(DPPH2) = 0.558, Q(ABTS)(2) = 0.534). The active site of sesaminol tended to be located on the hydroxyl group of the benzene ring (R1 position). A positive correlation between the bulky and positively charged groups at the 1H, 3H-furo [3, 4-c] furan group, the small, negatively charged groups at the R1 position and the antioxidant activity of sesaminol. This study provides an effective method to prepare sesaminol, reveals the structure-activity relationship of sesaminol and provides theoretical basis to design the novel compound.

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