Transcriptomic analysis reveals the gene expression profiles in the spleen of spotted knifejaw (Oplegnathus punctatus) infected by Vibrio harveyi
文献类型： 外文期刊
作者： Wang, Jie 1 ; Chen, Zhangfan 2 ; Xu, Wenteng 2 ; Li, Yangzhen 2 ; Lu, Sheng 2 ; Wang, Lei 2 ; Song, Yu 2 ; Wang, Na 2 ; Gong, Zhihong 2 ; Yang, Qian 1 ; Chen, Songlin 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China
3.Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
4.Shandong Key Lab Marine Fisheries Biotechnol & Gen, Qingdao 266071, Peoples R China
5.Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Shandong, Peoples R China
关键词： Oplegnathus punctatus; Comparative transcriptome; WGCNA; Vibro harveyi; Innate immunity; Spleen
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 2021影响因子：3.605； 五年影响因子：3.833 ）
ISSN： 0145-305X
年卷期： 2022 年 133 卷
收录情况： SCI
摘要： As one of the most valuable maricultured species, spotted knifejaw (Oplegnathus punctatus) has high popularity in eastern Asia. In recent years, diseases caused by Vibrio harveyi have brought huge economic losses in spotted knifejaw industry. To better understand the molecular mechanisms of immune response about V. harveyi resistance in spotted knifejaw, a comparative transcriptome analysis was performed on spleen tissues at five different time points post-infection (0, 12, 24, 48 and 72 hpi). A total of 4279 differentially expressed genes (DEGs) were identified. KEGG pathways analysis showed that multiple immune-related pathways were significant regulated, including Toll-like receptor signaling pathway, ECM-receptor interaction pathway, cytokine-cytokine receptor interaction pathway and hematopoietic cell lineage pathway. Weighted gene co-expression network analysis showed that several immune-related pathways of the highest correlation with 12 hpi (cor = 0.89, P = 7e-06) were significantly enriched. In addition, 12 hpi was a turning point for 7 gene clusters out of 9 that were divided according to gene expression patterns. Therefore, we speculated that 12 hpi might be a very critical time point for spotted knifejaw against V. harveyi infection. Additionally, qRT-PCR was carried out to validate the expressions of 12 DEGs. This study provided the first systematical transcriptome analysis of spotted knifejaw against V. harveyi. The results could help us better understand the dynamic immune responses of spotted knifejaw against bacterial infection, and provide useful information for antibacterial defense in spotted knifejaw industry as well.
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