文献类型： 外文期刊
作者： Huang, Guiyun 1 ; Gao, Fengying 2 ; Liu, Zhigang 2 ; Cao, Jianmeng 2 ; Chen, Gang 1 ; Lu, Maixin 2 ;
作者机构： 1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China
2.Pearl River Fisheries Res Inst CAFS, Key Lab Trop & Subtrop Fishery Resource Applicat, Xingyu Rd 1, Guangzhou 510380, Peoples R China
关键词： Misgurnus anguillicaudatus; 2b-RAD sequencing; Single nucleotide polymorphism (SNP); SNaPshot
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）
ISSN： 1877-7252
年卷期：
页码：
收录情况： SCI
摘要： The dojo loach Misgurnus anguillicaudatus is an endemic freshwater species in Asia. The effective conservation and molecular-aided selection of M. anguillicaudatus were limited without sufficient molecular markers. In this study, 112 novel single nucleotide polymorphism (SNP) markers were screened based on 2b-RAD sequencing database, and 56 SNPs of them were developed and characterized by genotyping 40 individuals using SNaPshot method. The observed heterozygosity (Ho) ranged from 0.025 to 0.675, while the expected heterozygosity (He) varied from 0.025 to 0.500. The minor allele frequency (MAF) ranged from 0.013 to 0.500. Among these SNPs, 18 loci were found to deviate significantly from the Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.05). The first set of SNP markers developed from M. anguillicaudatus will provide valuable information in further population genetic analysis and natural resource conservation.
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