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Target-site and non-target-site based resistance to clodinafop-propargyl in wild oats (Avena fatua L.)

文献类型: 外文期刊

作者: Han, Yujun 1 ; Sun, Ying 1 ; Ma, Hong 1 ; Wang, Ruolin 2 ; Lan, Yuning 2 ; Gao, Haifeng 3 ; Huang, Zhaofeng 2 ;

作者机构: 1.Northeast Agr Univ, Coll Plant Protect, Harbin 150030, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crop Northweste, Urumqi 830091, Peoples R China

关键词: Acetyl-CoA carboxylase (ACCase); Clodinafop-propargyl; Target-site resistance; Cross resistance; Metabolic resistance

期刊名称:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY ( 影响因子:4.7; 五年影响因子:4.7 )

ISSN: 0048-3575

年卷期: 2023 年 197 卷

页码:

收录情况: SCI

摘要: Wild oat (Avena fatua L.) is a common and problematic weed in wheat fields in China. In recent years, farmers found it increasingly difficult to control A. fatua using acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. The purpose of this study was to identify the molecular basis of clodinafop-propargyl resistance in A. fatua. In comparison to the S1496 population, whole dose response studies revealed that the R1623 and R1625 populations were 71.71- and 67.76-fold resistant to clodinafop-propargyl, respectively. The two resistant A. fatua populations displayed high resistance to fenoxaprop-p-ethyl (APP) and low resistance to clethodim (CHD) and pinoxaden (PPZ), but they were still sensitive to the ALS inhibitors mesosulfuron-methyl and pyroxsulam. An Ile2041-Asn mutation was identified in both resistant individual plants. The copy number and relative expression of the ACCase gene in the resistant population were not significantly different from those in the S1496 population. Under the application of 2160 g ai ha -1 of clodinafop-propargyl, the fresh weight of the R1623 population was reduced to 74.9%; however, pretreatment with the application of the cytochrome P450 inhibitor malathion and the GST inhibitor NBD-Cl reduced the fresh weight to 50.91% and 47.16%, respectively, which proved the presence of metabolic resistance. This is the first report of an Ile-2041-Asn mutation and probable metabolic resistance in A. fatua, resulting in resistance to clodinafop-propargyl.

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