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Overexpression of a Malus baccata CBF transcription factor gene, MbCBF1, Increases cold and salinity tolerance in Arabidopsis thaliana

文献类型： 外文期刊

作者： Liang, Xiaoqi 1 ; Luo, Guijie 2 ; Li, Wenhui 1 ; Yao, Anqi 1 ; Liu, Wanda 3 ; Xie, Liping 1 ; Han, Meina 1 ; Li, Xingguo 1 ; Han, Deguo 1 ;

作者机构： 1.Northeast Agr Univ, Coll Hort & Landscape Architecture, Natl Local Joint Engn Res Ctr Dev & Utilizat Small, Key Lab Biol & Genet Improvement Hort Crops Northe, Harbin 150030, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agr Sci, Suqian 223800, Peoples R China

3.Heilongjiang Acad Agr Sci, Hort Branch, Harbin 150040, Peoples R China

关键词： Malus baccata (L; ) Borkh; MbCBF1; Low temperature stress; High salt stress

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.437； 五年影响因子：5.731 ）

ISSN： 0981-9428

年卷期： 2022 年 192 卷

页码：

收录情况： SCI

摘要： CBFs play a crucial role when plants are in adverse environmental conditions for growth. However, there are few reports on the role of CBF gene in stress responses of Malus plant. In this experiment, a new CBF TF was separated from M. baccata which was named MbCBF1. MbCBF1 protein was found to be localized in the nucleus after subcellular localization. Furthermore, the expression of MbCBF1 was highly accumulated in new leaves and roots due to the high influence of cold and high salt in M. baccata seedlings. After introducing MbCBF1 into A. thaliana, transgenic A. thaliana can better adapt to the living conditions of cold and high salt. The increased expression of MbCBF1 in A. thaliana also increased the contents of proline, remarkablely improved the activities of SOD, POD and CAT, but the content of MDA was decreased. Although the chlorophyll content also decreased, it decreased less in transgenic plants. In short, above date showed that MbCBF1 has a positive effect on improving A. thaliana cold and high salt tolerance. MbCBF1 can regulate the expression of its downstream gene in transgenic lines, up -regulate the expression of key genes COR15a, RD29a/bandCOR6.6/47 related to low temperature under cold conditions and NCED3, CAT1, P5CS1, RD22, DREB2A,PIF1/4, SOS1 and SnRK2.4 related to salt stress under high salt conditions, so as to further improve the adaptability and tolerance of the transgenic plants to low temper-ature and high salt environment.

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